oriTDB

The information of the oriT region

oriTDB accession number	100278
Name	oriT_pYR1
Sequence Completeness	intact
oriT length	551 nt
oriT sequence	GAGCAGAGCTATGTGTGACAAGAAGTATAGAGATTACGAGGTAGCCATCATGGTCGATGT GAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACAT CCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTG CTACATCACAGACGGGATTAAGGCTAATCAGGAGCCTGTGATTTACCCGATCATTGAAGA AGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAGAAATATGAAGACCC GGCCAGAATTGGGGCATTTCTGGAAACCCTGATCACCGAAACCTGCCGGGCGTTGGAAGT GCAAATTGTCGATAGTGGCGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACT GTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCCCATGAGGATGAGAC CTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGCGTGAAAACTGTTTTAAG GAGAACCGACT
IR (inverted repeat)	IR1: [62-70] [71-79] nt (AACCCTTTC..GACAGGGTT) IR2: [143-150] [154-161] nt (TGGCCTGC..GGAGGCCA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L: 62-70;R:71-79;L:143-150;R:154-161

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	349
Name	TraI_pYR1
GenBank accession number	YP_001101767
Family	MOB_H
Length	992 aa
UniProt ID	A4IUA1
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 6.50E-102, Aligned region: 44..362
Note	putative relaxase
Protein sequence [Download]	MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQ ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES LHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREFLSKSGPSIMEAMLEAI SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVKTGKWKV NEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQE KGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQ KDAGPTESSKPDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLAND NPLQALKDVGGGLGDIDFPFDAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQE QNSLQGDDFPMFGGSDEPPSWAIEPLPMLTDAPEQPTHTPEMPHTDNVNQHEKDAKTLLV EMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSEVLSKLS HANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPG LDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKD INAEDFLPSGVTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGI SKHILCGMLSRAQRRPLLKKRQGKLYLEVNET

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	740
Plasmid name	pYR1
GenBank accession number	NC_009139.1
Incompatibility group	IncA/C2
Genome size	158038 bp
Coordinate of oriT [Strand]	54664..55214 [+]
Drug resistance	resistance to streptomycin, sulfonamide, tetracycline, and trimethoprim
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Yersinia ruckeri YR71 [29486]

Reference

[1] Welch TJ et al (2007) Multiple antimicrobial resistance in plague: an emerging public health risk. PLoS One. 2(3):e309. [PMID:17375195]