oriTDB

The information of the oriT region

oriTDB accession number	100275
Name	oriT_pAOVO02
Sequence Completeness	intact
oriT length	581 nt
oriT sequence	CCCTTGAGCGCGTGCGCCGTGTGCCGCGCGTAGGTCGCCACGGCTCGCCAGTAGGCGGCC ATCGGCCCCTTGCGGCGTGCCCATGCCTTCTCGGCTTCGTCGTTGGCCTGCGTGCGCAGC TCGCGCAGCACGCGGGCGAGGCGCTGCCGGTCGGCCACCGGCAGCGCCTGGAGTTCGCGG CCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCTCGTTTCTTG CCGGCGGTAGCCGGCCGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAGGCGCGAATA AGGGACAGCGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCGCCTATCCTGCCCGC CTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATCTGTCTGCATT CAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCTGGAAAGCGCGCTA GATGCAGCGGTGTCAGGCTGCTATCCACGCGCTTTACATAGACTTTCCACGGGTCGATAC ATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)	[139-151] [157-169] nt (AGGCGCTGCCGG..CCGGCAGCGCCT) [240-245] [250-255] nt (GCCGGC..GCCGGC) [308-326] [329-347] nt (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site	[355-356] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:139-151;R:157-169;L:240-245;R:250-255;L:308-326;R:329-347;D:355-356

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCCTTGAGCGCGTGCGCCGTGTGCCGCGCGTAGGTCGCCACGGCTCGCCAGTAGGCGGCC

61

ATCGGCCCCTTGCGGCGTGCCCATGCCTTCTCGGCTTCGTCGTTGGCCTGCGTGCGCAGC

121

TCGCGCAGCACGCGGGCGAGGCGCTGCCGGTCGGCCACCGGCAGCGCCTGGAGTTCGCGG

181

CCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCTCGTTTCTTG

241

CCGGCGGTAGCCGGCCGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAGGCGCGAATA

301

AGGGACAGCGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCGCCTATCCTGCCCGC

361

CTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATCTGTCTGCATT

421

CAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCTGGAAAGCGCGCTA

481

GATGCAGCGGTGTCAGGCTGCTATCCACGCGCTTTACATAGACTTTCCACGGGTCGATAC

541

ATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA

Reference
N/A

I. Information of Relaxase

ID	346
Name	TraI_pAOVO02
GenBank accession number	WP_011798731
Family	MOB_P
Length	752 aa
UniProt ID	A1WDT1
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-61, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIVKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAMSVQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGFAEHQRVSAVHNDTDNLHIHIA INKIHPTRHTMHEPFGQHRTLAELCTALERDYGLERDNHEPRQRGAEGRAADMERHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL SKPKLEARLGPFEPSPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA LEQARRRKDRLVEAAKRSNRLRRATIKVVGESRTNKKLLYAQASKALRDEIQAINKQYQQ DRQALYDSHSRRTWADWLKKEATHGNAEALAALRAREAAQGLQGNTIQGRGDARPGHAPV TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF KAQVIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP GGPGQRAAAHDDAARPVAIARAGFGRPGAGRTDAAGVHAGAVHRKPDIGRIGRVPPPQSQ HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRGISRPGTGVATAPPGLA AADKYIAEREAKRSKGFDIPKHARYTDGDGALAFQGVRNIEGQALALLKRGDEVMVMPID QATARRLSRIAVGDAVTITPKGSIKTSKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	737
Plasmid name	pAOVO02
GenBank accession number	NC_008766.1
Incompatibility group	IncP(Beta)
Genome size	63609 bp
Coordinate of oriT [Strand]	55155..55735 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidovorax sp. JS42 [232721]

Reference
N/A