oriTDB
The information of the oriT region
oriTDB accession number 100274
Name oriT_pNOCA01  insolico
Sequence Completeness intact
oriT length 510 nt
oriT sequence TTGAGGCCGCGGCCGCGAAGTCGCCGGCGGAACGCTGGGCCCCGCTGGCCCGCGAGGTCG ACCCGCGCCTGGCGGAGCAGGACGACTGGCCCGCCACCGCCGCGATGCTGCAACAGGCCC ACGAGCAAGGCCACGACGTCAGCGCCGCTACCCGCGCCCTGGTCGCAGCAGCACCCCTGG GCGACGCCCCCGCCCGCGAACTGCGCTACCGGCTGGTTCGGCAGGTTGGATTCGGCGAAC ACGACGCCGAATCCACCGACCGTGACGCCCCGAAGCGCCGGCGCGACGCGGGTACTGGAC ATGAGCTCGGCTCTTCGAACCCAGTGCACCGCAGCCGCCGCCGGTAGGCCTGGGCACAGG TTGAGACCTACAACGAGAGGGCTGTCGGACACTCAACGCGAGGTACCCGCGTGCAGACCT GCCACATTGCGGCCCACTGTGGGGCAGCGTCATGGCGCTCAAACGGGCGGGCTCCTTGTC CAGAGGTCTTCAAAGCGCCGGACTGGGCCG
IR (inverted repeat)IR1: [179-183] [185-189] nt  (GGGCG..CGCCC)
IR2: [225-237] [245-257] nt  (GTTGGATTCGGCG..CGCCGAATCCACC)
Location of nic site [155-156] nt
Conserved sequence flanking the
  nic site 		TACCCGCG|CCCT
Note predicted by the oriTfinder
L:179-183;R:185-189;L:225-237;R:245-257;D:155-156
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 345
Name Tra_pNOCA01 insolico
GenBank accession number WP_011751509
Family MOBF
Length 1967 aa
UniProt ID A1SC49
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.80E-91, Aligned region: 8..420
AAA_30 [PF13604.5], Evalue: 1.70E-54, Aligned region: 583..786
Toprim_N [PF08275.10], Evalue: 2.10E-27, Aligned region: 1523..1655
AAA_19 [PF13245.5], Evalue: 1.70E-08, Aligned region: 592..730
Toprim_2 [PF13155.5], Evalue: 7.50E-08, Aligned region: 1664..1751
Toprim_4 [PF13662.5], Evalue: 2.80E-05, Aligned region: 1662..1735
Note putative relaxase
Protein sequence [Download] MSLHKLTAGSGYDYLTRQVAAMDATDKGHTGLASYYTEKGETPGVWVGSGMEGIEGLDAG
DVVTADHMQNLFGAGHHPLATARTKELDLRVGRAGVGSPTAADYKAAARLGTPFKVYEND
VSAFRVEVAKRLAAVNEAAGLPGDWPVPAEERARIRTEIGRDFFRAEHGREPADARELAA
TIAKHSRPKTNAVAGYDLTFSPVKSVSTLWALADPKTAAVIEGAHQSAIKDALNFIETKA
LFTRQGTNGIRQVNVRGLLATAFTHRDSRAGDPDLHTHVAVANKVQTLDGKWLAIDGRLL
YKAKVSASETYNTALERHLGDALGVRFAERPNPDARKRPVREIVGVDPDLNHRFSKRRVS
VEARRKELAAQFQATHGRPPTPIETLQLAQRATLETREAKHEPRSLNDQREAWDREAVEV
LGSPRGLQQMLHGTLNPTAAGTAPVADAAWFAKTGDRIVTTMEGGRATWQYWHVYAEAQR
QVRAANVPTDQVSTVVGLLVSEVLDGRSVSITRPFDEISEPPELRKLDGASVYTVGGVHL
FTSSKVLAAEERLVEAAGRRDGYAVDEQNVALALLESEANGVTLNAGQATLVKEMATSGA
RLQLAIAPAGSGKTTAMRALAQAWTDGGGSVIGLAPSAAAADALRVSMGSQIDTQTDTLA
KLTHSLEQSQVTGAEVPDWVAGIGPQTLVVIDEAGMADTLSLDIAVQHVLERGGSVRLVG
DDQQLAAIGAGGVLRDIRATHGALQLTELVRFTDPAEGAASLALREGKGEALGFYLDRDR
VHVGDLATMTEDVFAAWQADRAAGLDAIMLAPTRELVSELNQQARAHRLEGADPASVGPA
RRLADGNEASIGELIITRTNDRKLRTSATDWVKNGDRWTVLGLHEGGDLTVQHTQHGRTV
RLPREYVETSTELGYACTVHTAQGVTTDTMHGLATGNESRQQLYTMLTRGKSGNHIYLQV
VGDGDPHSVIHPTLVRPLTPTDILESMLARDDAQRSATSLMREQADPATQLGEAAQRYLD
SLYVAAENHLRNTAGPDGSNVVEALDSTVDQLLPGLADEAAWPTLRAHLLLLGAAGENPV
EAMRAAASDRELVSAEDRAAVLDWRLDASGMRNVGTGPLPWIPGVPERLADDPHWGAYLD
QRAHLVDQLADEVRGRAAAQTSLPTWAQNGIRPDTDTVADVEVWRAAMQVPLDDRRPTGA
PQLQKASSTYQRRLNRRITGDHTPALTEWRQLLYSLAPQVRGDEFTPLLAERLAAMSRAG
VEAHQLLRTATAADTGAGPLPDEHAAAALWWRMARHLSPAVAAQVGDGSHGEGVTTSWAA
QLPELLSDERSARIQASAWWPALVSNIDHGLQRGWQLEALLSAGSALPSDDIDECQALVW
RTSIALEPIPDEHEHDDHFDEPPVDMWDGVEPDPATLVDHPDDTEWPLPHDLEADGDHLV
DHPEPDEAIDQVDVAPAAADNVDEQQFVEGDLTLAAYIRDLGGTPLEPTAADIRLMYQRA
DEWYSFPVTRERMIEINEITQTFFESRFTDSWGRDYLTGRFGIDLAGHEHFRPGQAPAGW
TNLVDHLRHRGVSGAEMLATGVATTASTGRLIDRFRDRVMFPVVHNGEVLGFVGRRRPEL
TDADQANGRGGPKYLNTADTPLFHKGAQLYGVVDELLAEGAVPVIVEGPMDAIAVTLASA
ARYVGVAPLGTSLTDEQASQLGGIGRDPIVATDADLAGEVAAERDFWMLTPHGLDPGYAR
FPEGLDPADLLAQRGPAALTASLASGQTLGDQLLSERLDNLAPEQARVAAMRVLAARPSR
AWAPGTNLVRARLKLSQLQSRRDLRDAIKAWDADPRRAARAELNSSSEVRARLEAAAAKS
PAERWAPLAREVDPRLAEQDDWPATAAMLQQAHEQGHDVSAATRALVAAAPLGDAPAREL
RYRLVRQVGFGEHDAESTDRDAPKRRRDAGTGHELGSSNPVHRSRRR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 736
Plasmid name pNOCA01
GenBank accession number NC_008697.1
Genome size 307814 bp
Coordinate of oriT  [Strand] 124416..124925 [+]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Nocardioides sp. JS614 [196162]
Reference
[1] Coleman NV et al (2011) Genome Sequence of the ethene- and vinyl chloride-oxidizing actinomycete Nocardioides sp strain JS614. J Bacteriol. 193(13):3399-400. [PMID:21551312]

Microbial Bioinformatics Group in MML, SJTU