oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100272
Name	oriT_pP99-018
Sequence Completeness	intact
oriT length	551 nt
oriT sequence	TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTG ACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCA GCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTAT TGAGG
IR (inverted repeat)	IR1: [62-70] [71-79] nt (AACCCTTTC..GACAGGGTT) IR2: [143-150] [154-161] nt (TGGCCTGC..GGAGGCCA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L: 62-70;R:71-79;L:143-150;R:154-161

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	343
Name	Relaxase_pP99-018
GenBank accession number	YP_908461
Family	MOB_H
Length	992 aa
UniProt ID	A0PAZ2
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 6.50E-102, Aligned region: 44..362
Note	putative relaxase
Protein sequence [Download]	MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQ ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES LHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREFLSKSGPSIMEAMLEAI SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVKTGKWKV NEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQE KGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQ KDAGPTESSKPDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLAND NPLQALKDVGGGLGDIDFPFDAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQE QNSLQGDDFPMFGGSDEPPSWAIEPLPMLTDAPEQPTHTPEMPHTDNVNQHEKDAKTLLV EMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSEVLSKLS HANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPG LDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKD INAEDFLPSGVTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGI SKHILCGMLSRAQRRPLLKKRQGKLYLEVNET

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	734
Plasmid name	pP99-018
GenBank accession number	NC_008612.1
Incompatibility group	IncA/C2
Genome size	150157 bp
Coordinate of oriT [Strand]	37699..38249 [+]
Drug resistance	resistance to kanamycin, chloramphenicol, tetracycline, and sulfonamide
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Photobacterium damselae subsp. piscicida PT99-018 [38294]

Reference

[1] Kim MJ et al (2008) Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp piscicida isolates collected in Japan and the United States. Antimicrob Agents Chemother. 52(2):606-11. [PMID:18070959]