oriTDB

The information of the oriT region

oriTDB accession number	100271
Name	oriT_pSK11-3
Sequence Completeness	intact
oriT length	463 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT AGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAA GTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
IR (inverted repeat)	IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-255] [268-281] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[290-291] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D: 290-291

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	342
Name	Relaxase_pSK11-3
GenBank accession number	WP_011669060
Family	MOB_P
Length	135 aa
UniProt ID	Q02VG5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-20, Aligned region: 16..124
Note	putative relaxase
Protein sequence [Download]	MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREM AILAGNQPKENERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKE HLHNVRPDRVLSQVV

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	733
Plasmid name	pSK11-3
GenBank accession number	NC_008505.1
Genome size	74750 bp
Coordinate of oriT [Strand]	29275..29737 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. cremoris SK11 [272622]

Reference

[1] Makarova K et al (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci U S A. 103(42):15611-6. [PMID:17030793]