oriTDB
The information of the oriT region
oriTDB accession number 100270
Name oriT_pMG828-3  insolico
Sequence Completeness intact
oriT length 82 nt
oriT sequence GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGCCATCAGTGCGGAT
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 341
Name pMG8283_03_pMG828-3 insolico
GenBank accession number YP_794134
Family MOBP
Length 521 aa
UniProt ID A0A1L2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.40E-19, Aligned region: 40..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFANKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRSSLSIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRDNAERRVQEARKICKQGCDIKRDENQRRYSPVHSFDRGITEKTPGRGE
RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDTGEPGRNQEAERESGEA
EREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGTGVTEHDGAGKTFAERI
RATATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVESAGAALERADRTLEPVI
QREQEIRDERLIQEREHALSLERERQLEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 732
Plasmid name pMG828-3
GenBank accession number NC_008488.1
Incompatibility group ColRNAI
Genome size 5219 bp
Coordinate of oriT  [Strand] 617..698 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli MG828 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU