oriTDB

The information of the oriT region

oriTDB accession number	100269
Name	oriT_pO86A1
Sequence Completeness	intact
oriT length	542 nt
oriT sequence	TTACTCTGGCCATAAGATAAAGCCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCC AGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCGGATTTACTGA GA
IR (inverted repeat)	[243-255] [257-269] nt (TATTTAATAATTC..GAATTATTAAATA) [311-318] [321-328] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[337-338] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:243-255;R:257-269;L:311-318;R:321-328;D:337-338

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTACTCTGGCCATAAGATAAAGCCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA

61

TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT

121

AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA

181

TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC

241

TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA

301

ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA

361

CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG

421

GTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCC

481

AGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCGGATTTACTGA

541

GA

Reference
N/A

I. Information of Relaxase

ID	340
Name	TraI_pO86A1
GenBank accession number	YP_788091
Family	MOB_F
Length	1756 aa
UniProt ID	Q08JE3
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 3.90E-76, Aligned region: 10..284 TraI [PF07057.10], Evalue: 9.10E-55, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.90E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 1.80E-08, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA IREAVGEGASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRAE IRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARVG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDSGQR TGTGSALMSMKEAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQSIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFNMVDLLTEAKSFAAEGTG CTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGET FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDCNGRSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREVARENLLQERLQQME RDMVHDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	731
Plasmid name	pO86A1
GenBank accession number	NC_008460.1
Incompatibility group	IncFIB(AP001918)
Genome size	120730 bp
Coordinate of oriT [Strand]	64739..65280 [-]
Drug resistance	resistance to neomycin, kanamycin, bleomycin, and streptomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli DIJ1 [562]

Reference
N/A