oriTDB

The information of the oriT region

oriTDB accession number	100266
Name	oriT_pRL8
Sequence Completeness	core
oriT length	50 nt
oriT sequence	GGATCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTGCTGAAGGG
IR (inverted repeat)	[22-27] [32-37] nt (CGTCGC..GCGACG)
Location of nic site	[11-12] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:22-27;R:32-37;D:11-12

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGATCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTGCTGAAGGG

Reference
N/A

I. Information of Relaxase

ID	337
Name	TraA_pRL8
GenBank accession number	WP_011654745
Family	MOB_Q
Length	1197 aa
UniProt ID	Q1M997
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 8.20E-75, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 2.70E-57, Aligned region: 385..574 AAA_19 [PF13245.5], Evalue: 1.10E-11, Aligned region: 390..517 Viral_helicase1 [PF01443.17], Evalue: 3.50E-05, Aligned region: 672..741
Note	putative relaxase
Protein sequence [Download]	MAVPHFSVSIVARGSGRSAVLSAAYRHCAKMEFEREARTIDYSRKQGLLHEEFVIPETAP DWLRSMIADRSVSGASEAFWNKVEAFEKRADAQLAKDVTIALPVELSNDQNIALVRDFVE RHITAKGMVADWVYHDALGNPHIHLMTTLRPLTEDGFGAKKVAVLAPAGKPVRNDAGKIV YELWAGSTDDFNAFRDGWFACQNRHLALAGLDIRIDGRSFEKQGIELTPTIHLGVGTKAI ERKGDNKTGWGEEKVALERLELQEERRAENARRIQRNPEIVLDLITREKSVFDERDIAKI LYRYIDDAALFQNLMARILQSPQTLRLDRERMDLVTGVRAPSKYTTRELIRLEAQMANQA IWLSQRSSHGVRHAVLSGVFSRHDRLSDEQKTAIEHVAGPERIAAVIGRAGAGKTTMMKA ARQAWEAAGYRVVGGALAGKAAEGLEKEAGIASRTLSAWELRWDQERDRLDEKSVFVLDE AGMVSSRQMARFVEAVTVSGAKLVLVGDPEQLQPIEAGAAFRAISGRIGYAELETIYRQR EQWMRDASLDLARGNVSAALDAYAQRDMVRTGWTRDEAITALIADWDHEYDPAKSTLILA HRRIDVRLLNEMARSKLVERGLIEAGHAFKTEDGTRQLAAGDQIVFLKNEGSLGVKNGML ARVVDAQPGRIVAEIGNGEDRRRVVVEQRFYANVDHGYATTVHKSQGATVDRVKVLASST LDRHLSYVAMTRHRETAELYVGLEEFAQRRGGVLIAHGEAPYEHKPGNRDSYYVTLGFAD GQERTVWGVDLARAMDASGARIGDRIGLKHVGSQRVTLPDGTEVDRNSWKVVPVEELAMA RLHERLSRPGSKETTLDYQDASHYRAALRFAEARSLHLMNVARTIAHDQLQWTIRQSSKL AELGARLVAVAAKLGLGGAKSTVSTTSAIKEAKPMVSGTTTFPRSIGQAAEDKLSADPGL KASWQEVSARFHHVFADPQAAFKTVNVDAMLANGTVAATTIVQIAEQPESFGALKGKTGL FAGSAEKRAHDTALVNAPALARDLQGFIAKRAEAASRYEDEERAVRTKLSLDIPALSASA KQVLERVRDAIDRNDIPAGLEFALADKMVKAELEGFAKAVSERFGERTFLPLAAKTADGK AFEVASAGMQPAQKNELRSAWDTIRTVQQLAAHERTAVALKQAEAIRQTQTKGLSLK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	728
Plasmid name	pRL8
GenBank accession number	NC_008383.1
Genome size	147463 bp
Coordinate of oriT [Strand]	126421..126470 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Rhizobium leguminosarum bv. viciae 3841 [216596]

Reference

[1] Young JP et al (2006) The genome of Rhizobium leguminosarum has recognizable core and accessory components. Genome Biol. 7(4):R34. [PMID:16640791]