oriTDB

The information of the oriT region

oriTDB accession number	100265
Name	oriT_pRB2256-F
Sequence Completeness	core
oriT length	36 nt
oriT sequence	GAGGGCGCGCTTATACGTCGTGCCGACGTGGCGTTT
IR (inverted repeat)	[15-20] [24-29] nt (ACGTCG..CGACGT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:15-20;R:24-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GAGGGCGCGCTTATACGTCGTGCCGACGTGGCGTTT

Reference
N/A

I. Information of Relaxase

ID	336
Name	TraA_pRB2256-F
GenBank accession number	WP_041384243
Family	MOB_Q
Length	961 aa
UniProt ID	_
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.00E-72, Aligned region: 17..217 AAA_30 [PF13604.5], Evalue: 4.20E-55, Aligned region: 346..535 PIF1 [PF05970.13], Evalue: 3.20E-07, Aligned region: 346..481 AAA_19 [PF13245.5], Evalue: 2.30E-07, Aligned region: 351..478
Note	putative relaxase
Protein sequence [Download]	MAIFHFSAKVIGRSSGRSAVAAAAYRAGEQLHDERIDRTHDFTNKAGVLHSEVMLPKGAP EALADRATLWNAVEAAEKRKDAQLAREVEFALPRELSKKDNIRLAREFVKAEFVEKGMIA DLNVHWDIGEDGKAKPHAHVMLTMREVTKDGFGAKVRDWNKTALIEQWRERWADHVNRAL AERDIDTRIDHRSLEAQGIALEPQDKIGPAASRIGGRGLEAERIEEHRAIAQRNGERIIA NPSLALDAITHQQATFTRRDLAAFVHRHSDGKEQFDAAYNAVRAAPELIALGKDGRGQDR FTSRAMIETEQRLHCAADAMAQRAGHAVNDVQRNAAFASAAKRGLVLSGEQKSAFEYVTK KGGLAVIVGYAGTGKSAMLGVAREAWESAGYNVRGAALSGITAEGLENGSGIASRTIASL EHQWGKGCEQLTAKDVLVIDEAGMVGTRQMERVLSHAAKSSAKLVLVGDQQQLQAIEAGA AFRAIHERHGGVEISEVRRQLSAWQQDTTRQLATGRTGEAIRTYEERGMVYATDTREVAR TELVERWDRERQDSPDERRIILTHTNDEVRELNQMAREKMRVAGALGADATIKAARGERK FASGDRIIFLRNERELGVKNGTLGTVAMSSPQRMAVRTDDGREVAFDTKYYAHIDHGYAA TIHKAQGMTVDRTHILATPGMDSHSAYVAMSRHREGLALHYGRDDFADQSKLVRTLSRER GKDMAGDYKPEQAFAELRGISFRERVMAMVRQVPEKAKSIFGNFRPQARQLEPLPAKSNT QNDQRRAVERYARALGDIGTMQAQGLPVLPHQKDALEKAGKALDAIRPHAATDLAKALER RPELIPEAAGGRSQEAMRAMNQEAAVRTDPALRSERFVSDWQGLSTARKQLEQQGDRAGA VRVSAKQNELAKSLERDPQVEGLLRGKTRELGIDPKPERSIAKELTATLARERTRAFDMG I

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	727
Plasmid name	pRB2256-F
GenBank accession number	NC_008036.1
Genome size	28543 bp
Coordinate of oriT [Strand]	4711..4746 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sphingopyxis alaskensis RB2256 [317655]

Reference

[1] Lauro FM et al (2009) The genomic basis of trophic strategy in marine bacteria. Proc Natl Acad Sci U S A. 106(37):15527-33. [PMID:19805210]