oriTDB
The information of the oriT region
oriTDB accession number 100261
Name oriT_pA1  insolico
Sequence Completeness intact
oriT length 591 nt
oriT sequence TGTCGTTACCCCTTGAGCGCGTGCGCCGTGTGCCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCGGCTTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCGAGGCGCTGCCGGTCGGCCGCCGGCAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGCCGGCGGTAGCCGGTCGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCAG GCGCGAATAAGGGACAGCGAAGATAGATAACCGGCTCGCCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)[148-159] [167-178] nt  (AGGCGCTGCCGG..CCGGCAGCGCCT)
[250-254] [259-263] nt  (CCGGC..GCCGG)
[317-334] [340-357] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [365-366] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:148-159;R:167-178;L:250-254;R:259-263;L:317-334;R:340-357;D:365-366
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 332
Name TraI_pA1 insolico
GenBank accession number YP_302615
Family MOBP
Length 755 aa
UniProt ID Q4AC74
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.70E-63, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRRTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMERHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEVRLGPFEPSPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LEQARRRKDRLVEAAKRSNRLRRATIKVVGESRTNKKLLYAQASKALRDEIQAINKQYQQ
DRQALYDSHSRRTWADWLKKEATHGNAEALAALRAREAAQGLQGNTIQGRGDARPGHAPV
TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF
KAQVIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTDRTERADQRPAEHRGRAG
RGPGGPGQRAAAHDDAARPVAIARAGFGRPGAGRTDAAGVHAGAVHRKPDIGRIGRVPPP
QSQHRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRGISRPGTGVATAPP
GLAAADKYIAEREAKRSKGFDIPKHARYTDGDGALAFQGVRNIEGQALALLKRGDEVMVM
PIDQATARRLSRIAVGDAVTITPKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 723
Plasmid name pA1
GenBank accession number NC_007353.2
Incompatibility group IncP(Beta)
Genome size 46557 bp
Coordinate of oriT  [Strand] 16444..17034 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Sphingomonas sp. A1 [90322]
Reference
[1] Harada KM et al (2006) Sequence and analysis of the 466-kb plasmid pA1 from Sphingomonas sp A1 that corresponds to the typical IncP-1beta plasmid backbone without any accessory gene. Plasmid. 56(1):11-23. [PMID:16445980]

Microbial Bioinformatics Group in MML, SJTU