oriTDB

The information of the oriT region

oriTDB accession number	100259
Name	oriT_pSHaeC
Sequence Completeness	intact
oriT length	238 nt
oriT sequence	AGTGGCTAACAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATT ATCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAATGCTTGCCAAA CCACTTTATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATA TCTTTCGAAGAAAGCGTGGTGGGATTTGATGAGCGAACAAAATAAATTTGTGGCTAGC
IR (inverted repeat)	IR1: [52-59] [61-68] nt (TTGGGGAT..ATCCCCAA) IR2: [94-98] [104-108] nt (TGGCT..AGCCA)
Location of nic site	[115-116] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	predicted by the oriTfinder
	L:52-59;R:61-68;L:94-98;R:104-108;D:115-116

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGCTAACAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATT

61

ATCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAATGCTTGCCAAA

121

CCACTTTATTACTTGATGAAATTTAGATTATCATATAATCTAAATTTATATTAAGTAATA

181

TCTTTCGAAGAAAGCGTGGTGGGATTTGATGAGCGAACAAAATAAATTTGTGGCTAGC

Reference
N/A

I. Information of Relaxase

ID	330
Name	Rlx_pSHaeC
GenBank accession number	WP_011276930
Family	MOB_P
Length	335 aa
UniProt ID	Q4L2X5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.10E-80, Aligned region: 8..242
Note	putative relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKATRELYGKTGGNEGHVIIQ SFKPGEVTPEQCNQLGLALAEKFATNHQVAVYTHCDTDHIHNHIVINAVNLETGKKFNNN KQALRDLRDFNDNVCREHGLSVPEKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAI DQSKATNMDEFKEHLSQYGIEIARVTPKSITYRHLAEDKKVRGRRLGEDYNKGGIEDGFE RQIQRQQQEREYDKDIQPKRPTSNRDEPAQRDTGVTQSDWDKFAQDTNELERRRQAAESA RLVDEKARRDREERERAEQASRTIIDNDRDHGLEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	721
Plasmid name	pSHaeC
GenBank accession number	NC_007171.1
Genome size	8180 bp
Coordinate of oriT [Strand]	5700..5937 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus haemolyticus JCSC1435 [279808]

Reference

[1] Takeuchi F et al (2005) Whole-genome sequencing of staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species. J Bacteriol. 187(21):7292-308. [PMID:16237012]