oriTDB

The information of the oriT region

oriTDB accession number	100256
Name	oriT_pCCK381
Sequence Completeness	core
oriT length	89 nt
oriT sequence	GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGTAAAATGACGGTCGGGGTTT GCGGTGTCTGTGCTCCGATGATAGCATAA
IR (inverted repeat)	[5-10] [16-21] nt (GTTTCT..AGAAAC)
Location of nic site	[32-33] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:5-10;R:16-21;D:32-33

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCCGTAAAATGACGGTCGGGGTTT

61

GCGGTGTCTGTGCTCCGATGATAGCATAA

Reference
N/A

I. Information of Relaxase

ID	327
Name	Mob_pCCK381
GenBank accession number	YP_232869
Family	MOB_Q
Length	703 aa
UniProt ID	Q50I79
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 6.00E-48, Aligned region: 394..641 MobA_MobL [PF03389.14], Evalue: 4.10E-34, Aligned region: 16..218
Note	putative relaxase
Protein sequence [Download]	MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDG ADLYERANGRLFKEIEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPH CHLMISERKNDGIERPADQWFKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRA LEQAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMESRGIRTERADIALAIDTANGQIID LQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTGGRSPAADRRGTAGQRGAGRE LDEPATASHERMAAGSKGHEGSRRGTGQGRPDGPERRPWLDMEVVGRGLDRFRDAYSGAA DRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSSDDLDAMKQEGREPA AIIETSPKNYQAWVKVAQDAPADHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDK YTSRTGYQPWVLCRESSGKSATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRY RRSVVDDYRSEMAGLVKRYGDDLSKCDFIAAMKLASKGREPDEISKAMAEASPAIMERKA GHEADYIKRTVQKVMELPQVQEARAELAKQTQKQRSKGPDLSM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	718
Plasmid name	pCCK381
GenBank accession number	NC_006994.1
Incompatibility group	IncQ1
Genome size	10874 bp
Coordinate of oriT [Strand]	812..900 [-]
Drug resistance	resistance to chloramphenicol and florfenicol
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pasteurella multocida 381 [747]

Reference

[1] Kehrenberg C et al (2005) Plasmid-borne florfenicol resistance in Pasteurella multocida. J Antimicrob Chemother. 55(5):773-5. [PMID:15814600]