 oriTDB accession number | 100255 |
| Name | oriT_pC221  |
Sequence Completeness  | core |
| oriT length | 53 nt |
| oriT sequence | |
| IR (inverted repeat) | [12-19] [35-42] nt (GTATATTC..GAATATAC) |
| Location of nic site | [50-51] nt |
| Conserved sequence flanking the nic site | CGCTTG|CCA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 326 |
| Name | pC221_004_pC221 |
| GenBank accession number | YP_232728 |
| Family | MOBP |
| Length | 315 aa |
| UniProt ID | A9CQY2 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 5.20E-79, Aligned region: 8..239 |
| Note | putative relaxase |
| Protein sequence [Download] | MATTKLGNTKSASRAINYAEKRAEEKSGLNCDVDYAKSAFKQTRALYGKEDGIQAHTVIQ SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHTDKDHYHNHIVINSVDLETGKKYQSN KKQRDLVKKENDNICREHGLSVTERGIAKMRYTQAEKGIVFDRDEYSWKDELRDLIENAK THTSNLETFSEHLEEKGVGVKLRGETISYKPENENKWVRGRTLGSEYEKGAIDHEHERHQ KQQREPEYADEFKINWDAVEQHTEQLKQRRVERAQETKQAHSKISSRDTRESENQRERAK GNNIRIERGDEGLSR |
Reference| ID | 717 |
| Plasmid name | pC221 |
| GenBank accession number | NC_006977.1 |
| Genome size | 4555 bp |
| Coordinate of oriT [Strand] | 3101..3153 [+] |
| Drug resistance |  chloramphenicol resistance |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Staphylococcus aureus [1280] |
Reference| [1] Brenner DG et al (1985) The use of synthetic oligonucleotides with universal templates for rapid DNA sequencing: results with staphylococcal replicon pC221. EMBO J. 4(2):561-8. [PMID:3860383] |
| [2] Shaw WV et al (1985) Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221 Nucleotide sequence analysis and expression studies. FEBS Lett. 179(1):101-6. [PMID:3855295] |