 oriTDB accession number | 100252 |
| Name | oriT_pAPEC-O2-R  |
Sequence Completeness  | intact |
| oriT length | 462 nt |
| oriT sequence | AAGAAGAGCACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGTACTGACCCTAAC AAGGGTCGTCATAGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATTTATAACATAAGCTAATGATTCAAAAAACAAATCAGCAAAAACTT GTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTT GTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGG ATGTTAGCCATCTGCTTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGAT CTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA |
| IR (inverted repeat) | [142-151] [154-163] nt (ATAGGGTCGT..ACGACCCTAT) [231-238] [241-248] nt (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [257-258] nt |
| Conserved sequence flanking the nic site | GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 323 |
| Name | TraI_pAPEC-O2-R |
| GenBank accession number | YP_190115 |
| Family | MOBF |
| Length | 1756 aa |
| UniProt ID | Q5JBP8 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.30E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 7.60E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 5.10E-34, Aligned region: 968..1159 AAA_19 [PF13245.5], Evalue: 7.70E-09, Aligned region: 973..1108 |
| Note | putative relaxase |
| Protein sequence [Download] | MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE TRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGINVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVTSVSEDAMTVVVPGRAEPASLPVADSPFTALKLENGWVETPGH SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTS FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD |
Reference| ID | 714 |
| Plasmid name | pAPEC-O2-R |
| GenBank accession number | NC_006671.1 |
| Incompatibility group | IncFII(pRSB107) |
| Genome size | 101375 bp |
| Coordinate of oriT [Strand] | 39494..39955 [+] |
| Drug resistance |  resistance to ampicillin, sulfisoxazole, tetracycline, streptomycin,gentamicin, trimethoprim |
| Heavy-metal resistance | resistance to silver nitrate |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli A2363 [562] |
Reference| [1] Johnson TJ et al (2005) DNA sequence and comparative genomics of pAPEC-O2-R, an avian pathogenic Escherichia coli transmissible R plasmid. Antimicrob Agents Chemother. 49(11):4681-8. [PMID:16251312] |