oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100250
Name	oriT_pRK2
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	321
Name	MobA_pRK2
GenBank accession number	YP_052693
Family	MOB_P
Length	517 aa
UniProt ID	Q6GUF9
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.80E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAERRIQQARRICQRGCDLKRDENQRRYSPVYGLDGGIAEKTPGRGE RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRVGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTELERADRTLEPVIQREL EIREERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	712
Plasmid name	pRK2
GenBank accession number	NC_005970.1
Genome size	5360 bp
Coordinate of oriT [Strand]	1049..1223 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ATCC 9637 [562]

Reference

[1] Stepánek V et al (2005) Cryptic plasmid pRK2 from Escherichia coli W: sequence analysis and segregational stability. Plasmid. 54(1):86-91. [PMID:15907542]

[2] Sobotková L et al (1999) Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W. Folia Microbiol (Praha). 44(3):263-6. [PMID:10664880]