oriTDB
The information of the oriT region
oriTDB accession number 100249
Name oriT_pSK639  insolico
Sequence Completeness intact
oriT length 238 nt
oriT sequence TGGCTAGCATTTAAATCACTCATTTTTTATGTAAGAAGAAACATAAGGGGCTTGGGGATT ATCCCCAACAAGCAGGCGACACTACCACGTAAGTGGCTAGCAAAGCCAATGCTTGCCAAA CCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAATGTAAGAAAGGCGTT ATCTTTCGAAGAAAGCGTGGTGGGAATAATGAGCGAACAAAACACATTTGTGGCTAGC
IR (inverted repeat)IR1: [52-59] [61-68] nt  (TTGGGGAT..ATCCCCAA)
IR2: [94-98] [104-108] nt  (TGGCT..AGCCA)
Location of nic site [115-116] nt
Conserved sequence flanking the
  nic site 		TGCTTG|CCA
Note predicted by the oriTfinder
L:52-59;R:61-68;L:94-98;R:104-108;D:115-116
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 320
Name Orf337_pSK639 insolico
GenBank accession number NP_976278
Family MOBP
Length 337 aa
UniProt ID P95738
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 3.50E-83, Aligned region: 9..243
Note putative relaxase
Protein sequence [Download] MMATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVII
QSFKPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNN
NKQALRNVRNFNDEVCMEHGLSVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREA
IDQSQATNMDEFKDHLNQHGIEIERVTPKSITYRHLAEDKKVRGRKLGEDYNKGGIEDGF
ERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQADWDQFAQDTNELERSRQAAE
SARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 711
Plasmid name pSK639
GenBank accession number NC_005566.1
Genome size 8013 bp
Coordinate of oriT  [Strand] 173..410 [+]
Drug resistance experimental trimethoprim resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus epidermidis SK398 [1282]
Reference
[1] Apisiridej S et al (1997) Molecular analysis of a mobilizable theta-mode trimethoprim resistance plasmid from coagulase-negative staphylococci. Plasmid. 38(1):13-24. [PMID:9281492]

Microbial Bioinformatics Group in MML, SJTU