oriTDB

The information of the oriT region

oriTDB accession number	100248
Name	oriT_pS194
Sequence Completeness	core
oriT length	53 nt
oriT sequence	CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA
IR (inverted repeat)	[12-19] [35-42] nt (GTATATTC..GAATATAC)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	CGCTTG\|CCA
Note	predicted by the oriTfinder
	L:12-19;R:35-42;D:50-51

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

Reference
N/A

I. Information of Relaxase

ID	319
Name	ORF(rlx)_pS194
GenBank accession number	NP_976272
Family	MOB_P
Length	320 aa
UniProt ID	P12054
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.20E-78, Aligned region: 8..238
Note	putative relaxase
Protein sequence [Download]	MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQ SFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSN KEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDN AVAFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKLGDSYDKGVIENGFAIEKF RREREEEREYDEYADTFEVDWDAFAENSEDLRKRRIARTEETKQASNKIYIRDERTTGLE RKGIAGNQVEFEKDDGGLSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	710
Plasmid name	pS194
GenBank accession number	NC_005564.1
Genome size	4397 bp
Coordinate of oriT [Strand]	902..954 [+]
Drug resistance	streptomycin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] Wang PZ et al (1993) Origin recognition specificity in pT181 plasmids is determined by a functionally asymmetric palindromic DNA element. EMBO J. 12(1):45-52. [PMID:8428593]

[2] Projan SJ et al (1988) Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus. Nucleic Acids Res. 16(5):2179-87. [PMID:3357770]