 oriTDB accession number | 100248 |
| Name | oriT_pS194  |
Sequence Completeness  | core |
| oriT length | 53 nt |
| oriT sequence | CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA |
| IR (inverted repeat) | [12-19] [35-42] nt (GTATATTC..GAATATAC) |
| Location of nic site | [50-51] nt |
| Conserved sequence flanking the nic site | CGCTTG|CCA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 319 |
| Name | ORF(rlx)_pS194 |
| GenBank accession number | NP_976272 |
| Family | MOBP |
| Length | 320 aa |
| UniProt ID | P12054 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 3.20E-78, Aligned region: 8..238 |
| Note | putative relaxase |
| Protein sequence [Download] | MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQ SFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSN KEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDN AVAFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKLGDSYDKGVIENGFAIEKF RREREEEREYDEYADTFEVDWDAFAENSEDLRKRRIARTEETKQASNKIYIRDERTTGLE RKGIAGNQVEFEKDDGGLSR |
Reference| ID | 710 |
| Plasmid name | pS194 |
| GenBank accession number | NC_005564.1 |
| Genome size | 4397 bp |
| Coordinate of oriT [Strand] | 902..954 [+] |
| Drug resistance |  streptomycin resistance |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Staphylococcus aureus [1280] |
Reference| [1] Wang PZ et al (1993) Origin recognition specificity in pT181 plasmids is determined by a functionally asymmetric palindromic DNA element. EMBO J. 12(1):45-52. [PMID:8428593] |
| [2] Projan SJ et al (1988) Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus. Nucleic Acids Res. 16(5):2179-87. [PMID:3357770] |