oriTDB

The information of the oriT region

oriTDB accession number	100245
Name	oriT_pPSR1
Sequence Completeness	intact
oriT length	146 nt
oriT sequence	CGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGT ATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCGGAGCGGGAAAGCGTTG TCGTTGTCGTTGTTGGTTGTTGTCAT
IR (inverted repeat)	IR1: [38-43] [52-57] nt (AAAAAG..CTTTTT) IR2: [72-83] [87-98] nt (GTGCCAAAGGGG..CCCCTTTGAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:38-43;R:52-57;L:72-83;R:87-98

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGAATTCGCTAAACAGGCACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGT

61

ATCCAATCGACGTGCCAAAGGGGATACCCCTTTGAAACCCCGCGGAGCGGGAAAGCGTTG

121

TCGTTGTCGTTGTTGGTTGTTGTCAT

Reference
N/A

I. Information of Relaxase

ID	316
Name	Orf54_pPSR1
GenBank accession number	NP_940739
Family	MOB_P
Length	1249 aa
UniProt ID	Q6VE73
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.60E-07, Aligned region: 10..266
Note	putative relaxase
Protein sequence [Download]	MLIRVSGYNTGAQEYLEQGNKSGREFTRDELDHRLIIEGQLSVTRAIYESIPDHGQDRYL TFTLSFKEDTVSPELLKAVTTDFKNFFMHAYKPEEFNLYAEAHLPKMKTVTDRKTGEVID RKPHIHIIIPRINLLSGNEANPVDVYKNHEKYFEAFQEHINQKYGLSSPRENVRADITDA ASVLSRYKGDDFYGKNRQFKQQLVKQVIERGVTSRADFYALVAEHGETRIRNEGKDNEYI SVKLPGDAKGTNLKDTIFQDDFIVRRELRKPPLEASVIQERLLAWPQRAREIKYVNKATP KFRKQYSQASPEERVRLLAERETNFYRAHGEDYETVHTGQRQRDHQRSPAETTGRRTAAP ADGVQDLSVSDVADHRQAGPTRSRDGALLLPSDAHVHVGQSQPGGDSGLRSSVPGGGRGR RAGSTAERGRGGRQPAAVSQETKGTATPAGATGRRRAGAGKPRNTRVRAGRVVPPYAQNP HRVATIADIEERGRRLFDPLKRPSDNALVFYRSSSHPQVADQLPQARAATTTPVQSTAGR RPRSSGKPRPPRQWRPGAVPPYAKNPHRVATVADIEQRARMLFDPLKRPADKVLVFKRAS IKALTVNKHASTVAAYFTRQAQHNQIAPAHRRAIRRIDQQYFALRRAVFSDQRLTRQDKA QLVSVLTFERLKAREQFHNPKPNIEVNLMGSAAIRNLLDDEKEDPGFSISGARGPGPEGV RERVKRVMDRFAKQVDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGT TISMRRTGITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKSMN QSLADRRAELDIERDGQSIGPATDLPRHVDDATRDVRDQADRLGATVPIEALYGQGKTAD QVSQALTAQLDTVPESERIAFVETVAITLGIPERGQPKGDEAFAQWQAQRAQPAANSVAS ATSEAQANVPTPSDAVPEPVNPEAAKPALANDPDLQSPSELVRLEAQWRRDFPMSEADVR ASDTVMGLRGEDHAIWIIATDDKTPEAAALLTAYMENDSYREAFKASIVAAYKQVENSPQ SVDDLDHLTAMAAQIVNEVEERLFPTPQAATGQTAPSRSKVIEGTLIDHGEAPYQHNDDN QMSYFVTLKPEGGKPRTVWGVGLEEAMSDADLKQGDQVRLQDLGTQPVVVQVLEEDGTVT DKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMNMD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	707
Plasmid name	pPSR1
GenBank accession number	NC_005205.1
Genome size	72601 bp
Coordinate of oriT [Strand]	66771..66916 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas syringae pv. syringae A2 [321]

Reference

[1] Sundin GW et al (2004) Complete nucleotide sequence and analysis of pPSR1 (72,601 bp), a pPT23A-family plasmid from Pseudomonas syringae pv syringae A2. Mol Genet Genomics. 270(6):462-76. [PMID:14634868]