oriTDB

The information of the oriT region

oriTDB accession number	100244
Name	oriT_pSCFS1
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CTTTATGGAATAAAGTATAGTGTGTTATACTTTACATG
IR (inverted repeat)	[1-6] [10-15] nt (CTTTAT..ATAAAG)
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:1-6;R:10-15;D:24-25

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTTATGGAATAAAGTATAGTGTGTTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	315
Name	ORF5_pSCFS1
GenBank accession number	NP_899168
Family	MOB_V
Length	392 aa
UniProt ID	Q7WVB7
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 2.40E-69, Aligned region: 39..230
Note	putative relaxase
Protein sequence [Download]	MQGFVAEPIASQSTHFMDIKYSLLYFTWKLTELWGCIFVSFAVVRMQKMKSPDLKGMQFH NQRERESRTNPDIDPDRAHFNYDLLHQEKIDYNKQVKAIIESQKVSERKTRKDAVLVNEL LVTSDRKFFDGLDPAEQKRFFEESYKLFSERYGKQNIAYATVHNDEKTPHMHLGVVPMRD GKLQGKNIFNRQELQWMQEEFPKHMQSVGFEVERGIASDRKHIEMSRFKALTLNEEIKTL EKETEVLRNALTASKKVDELQVSKPSLFDRNHVKLPVEDFEALKARAKATEAIESTIEAH EKRFDDMVDNVIASDRKLDQEKTKTAQLQKENSGLKKENLELQKENKTLKSQLNVLMEFA KSQLEKFKEWQKERQQEKEKNIARKRDQELER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	706
Plasmid name	pSCFS1
GenBank accession number	NC_005076.1
Genome size	17108 bp
Coordinate of oriT [Strand]	15358..15395 [-]
Drug resistance	resistance to florfenicol,chloramphenicol, MLS_B(macrolidelincosamide-streptogramin B), spectinomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus sciuri [1296]

Reference

[1] Kehrenberg C et al (2004) Nucleotide sequence and organization of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri. J Antimicrob Chemother. 54(5):936-9. [PMID:15471995]