oriTDB

The information of the oriT region

oriTDB accession number	100243
Name	oriT_pLW043
Sequence Completeness	core
oriT length	36 nt
oriT sequence	CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
IR (inverted repeat)	[4-10] [14-20] nt (GCGAACG..CGTTCGC)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCGCC\|CT
Note	predicted by the oriTfinder
	L:4-10;R:14-20;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC

Reference
N/A

I. Information of Relaxase

ID	314
Name	nicking enzyme_pLW043
GenBank accession number	NP_878032
Family	MOB_Q
Length	665 aa
UniProt ID	O87361
PDB ID	4HTE
Pfam	MobA_MobL [PF03389.14], Evalue: 7.70E-55, Aligned region: 17..218
Note	putative relaxase
Protein sequence [Download]	MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKH LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGKKSSISKHNESIKKRNQQKI DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYKENERLYDLKQKTS LITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSDDEKIFITRTI LNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAF NEVTDIKEKQQLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN KNQHDKFVFGAIQKKLLSEYDFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILV DKETYNNYNRKQQAYGLINAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK RGMHL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	705
Plasmid name	pLW043
GenBank accession number	NC_005054.1
Genome size	57889 bp
Coordinate of oriT [Strand]	54976..55011 [+]
Drug resistance	resistance to vancomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] Weigel LM et al (2003) Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science. 302(5650):1569-71. [PMID:14645850]