oriTDB

The information of the oriT region

oriTDB accession number	100242
Name	oriT_pLG13
Sequence Completeness	intact
oriT length	82 nt
oriT sequence	GGGTGTCGGGGCGCAGCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAA CCATGCGGCATCAGTGCGGATT
IR (inverted repeat)	[3-10] [17-24] nt (GTGTCGGG..CCTGACCC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-10;R:17-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAA

61

CCATGCGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	313
Name	MobA_pLG13
GenBank accession number	NP_863577
Family	MOB_P
Length	517 aa
UniProt ID	Q9F899
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-19, Aligned region: 41..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFIIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLSDNLPETKQALAESVTRWIDA LYHVGEIKGRQDVIQALTEAGIEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDREIAGKTQGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAQT EREDMGRGVCAGREQEISGPSVREISRGDSLSGERVGTSEGVTQNDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHALSLERERQLEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	704
Plasmid name	pLG13
GenBank accession number	NC_005019.1
Incompatibility group	ColRNAI
Genome size	6293 bp
Coordinate of oriT [Strand]	834..915 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli LG74 [562]

Reference
N/A