oriTDB
The information of the oriT region
oriTDB accession number 100241
Name oriT_pAMalpha1  insolico
Sequence Completeness intact
oriT length 261 nt
oriT sequence CTGCTAGCCGGTGCTAGTAATGTGCTAATTTGGTGCTAAAAACCTGCTAGTCACTGCTAG GAATCTGCTAGTTTGGGTGCTAGAAACCTGCTATTGAGTGCTAAAAATGTGCTAGTTTAC CTGAAAGTACCTGCTAGTAAAGTGCTAGCGAAGAACGAAAGTGTACCCCACGGGAAAGCC TTGCACTGCAAGGCGGGGCACCTTGTGTGCCCCCCAGTTCCCCTTATGCTCTTCCGAAAC ACAGGAAGAAATTAAGCGAAT
IR (inverted repeat)IR1: [178-185] [187-194] nt  (GGGGCAC.GTGCCCC)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note predicted by the oriTfinder
L:178-185;R:187-194;L:195-201;R:207-213
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 312
Name MobB_pAMalpha1 insolico
GenBank accession number NP_863352
Family MOBV
Length 420 aa
UniProt ID Q7B6N6
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note putative relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT
GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK
KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK
ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 703
Plasmid name pAMalpha1
GenBank accession number NC_005013.1
Genome size 9759 bp
Coordinate of oriT  [Strand] 3553..3813 [+]
Drug resistance experimental tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecalis [1351]
Reference
[1] Francia MV et al (2002) Amplification of the tetracycline resistance determinant of pAMalpha1 in Enterococcus faecalis requires a site-specific recombination event involving relaxase. J Bacteriol. 184(18):5187-93. [PMID:12193637]