oriTDB

The information of the oriT region

oriTDB accession number	100240
Name	oriT_pSE-12228-02
Sequence Completeness	core
oriT length	53 nt
oriT sequence	CCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA
IR (inverted repeat)	[12-19] [35-42] nt (GTATATTC..GAATATAC)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	CGCTTG\|CCA
Note	predicted by the oriTfinder
	L:12-19;R:35-42;D:50-51

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCATCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

Reference
N/A

I. Information of Relaxase

ID	311
Name	RLX_pSE-12228-02
GenBank accession number	NP_863255
Family	MOB_P
Length	283 aa
UniProt ID	A0A0H2VL15
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.80E-81, Aligned region: 8..235
Note	putative relaxase
Protein sequence [Download]	MATTKLGNTKSASRAINYAEKRAEEKSGLNCDIDYAKSSFKQTRALYGKENGVQAHTVIQ SFKPGEVTPEQCNQLGLELAEKIAPNHQVAVYTHADTNHVHNHIVINSIDLETGKKYQSN KKQREFVKQKNDEVCREHGLSVTERNATMRYTQAEKGLIEKDKSSWKDEIRDAIEQSQAT NFEELERELNQLDITIDRVTNKTITYRHLKENKKVRGKNLGEQFDKGALENEFAVANERR EFEQQLSEYEPRESEYRPRDTEKNIKRPKELRVQSRENDFELE

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	702
Plasmid name	pSE-12228-02
GenBank accession number	NC_005007.1
Genome size	4679 bp
Coordinate of oriT [Strand]	3054..3106 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus epidermidis ATCC 12228 [176280]

Reference

[1] Zhang YQ et al (2003) Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol Microbiol. 49(6):1577-93. [PMID:12950922]