oriTDB

The information of the oriT region

oriTDB accession number	100239
Name	oriT_pSE-12228-06
Sequence Completeness	intact
oriT length	240 nt
oriT sequence	AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGAT TACCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAA ACCACTTAACGACTTGATGAATCTTACTTTATCATATAAAGTAAGATGTAAGAACGGCGT TATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC
IR (inverted repeat)	IR1: [1-8] [14-21] nt (AGTGGCTA..TAGCCACT) IR2: [22-29] [34-41] nt (ATTTTTTC..GAAAAAAT) IR3: [95-99] [105-109] nt (TGGCT..AGCCA)
Location of nic site	[116-117] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	predicted by the oriTfinder
	L:1-8;R:14-21;L:22-29;R:34-41;L:95-99;R:105-109;D:116-117

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCATAAGGGGCTTGGGGAT

61

TACCCCCAACAAGCAGGCGCGTCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAA

121

ACCACTTAACGACTTGATGAATCTTACTTTATCATATAAAGTAAGATGTAAGAACGGCGT

181

TATCTTTCGAAGAAAGCGTGGTGGGATTGAATGAGCGAACAAAACACATTTGTGGCTAGC

Reference
N/A

I. Information of Relaxase

ID	310
Name	Mob_pSE-12228-06
GenBank accession number	NP_863197
Family	MOB_P
Length	336 aa
UniProt ID	A0A0H2VJA7
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-79, Aligned region: 8..242
Note	putative relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARELYGKTDGNQGHVIIQ SFQPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHADTNHVHNHIVLNSIDLNTGKKFNNN KQALRDVRAFNDEVCRAQGLSVPEKDTARLRYTQTEKAIADPNTTSTAQYSWKDDIREAI DQSTATNMEEFQKHLNQHGITIERVTPKSITYRHLAENKKVRGRKLGEDYNKGGIQHGFE RQIQRRQQQKHKSDTNFQPKRSTSNRHESTERDTGVTQSDWDQFAQDTNELERRRQAAES ARLVDEKARRDREKRVREKPTSRPIINIDRDHGLEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	701
Plasmid name	pSE-12228-06
GenBank accession number	NC_005003.1
Genome size	6585 bp
Coordinate of oriT [Strand]	1267..1506 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus epidermidis ATCC 12228 [176280]

Reference

[1] Zhang YQ et al (2003) Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol Microbiol. 49(6):1577-93. [PMID:12950922]