oriTDB

The information of the oriT region

oriTDB accession number	100237
Name	oriT_pAH82
Sequence Completeness	intact
oriT length	459 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTACAGTATTTAT GGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAA GTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC
IR (inverted repeat)	IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-255] [268-281] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[290-291] nt
Conserved sequence flanking the nic site	CTTA\|CA
Note	predicted by the oriTfinder
	L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D:290-291

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA

121

AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA

181

GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC

241

AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTACAGTATTTAT

301

GGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTA

361

CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAA

421

GTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC

Reference
N/A

I. Information of Relaxase

ID	308
Name	Mob_pAH82
GenBank accession number	NP_862609
Family	MOB_P
Length	135 aa
UniProt ID	Q9FB66
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.40E-20, Aligned region: 16..125
Note	putative relaxase
Protein sequence [Download]	MTVIYMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTM AIALKNKPRKNEIYGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGNSAEFIIATHTDKP HLHNVRPDRVLSQVV

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	699
Plasmid name	pAH82
GenBank accession number	NC_004966.1
Genome size	20331 bp
Coordinate of oriT [Strand]	4264..4722 [+]
Drug resistance	_
Heavy-metal resistance	cadmium resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis [1360]

Reference

[1] O' Sullivan D et al (2001) Naturally occurring lactococcal plasmid pAH90 links bacteriophage resistance and mobility functions to a food-grade selectable marker. Appl Environ Microbiol. 67(2):929-37. [PMID:11157264]

[2] O'Sullivan D et al (2000) Novel type I restriction specificities through domain shuffling of HsdS subunits in Lactococcus lactis. Mol Microbiol. 36(4):866-75. [PMID:10844674]