oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100236
Name	oriT_pBLO1
Sequence Completeness	core
oriT length	32 nt
oriT sequence	ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)	[1-8] [12-19] nt (ATGCAACC..GGTTGCAT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:1-8;R:12-19;D:28-29

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	307
Name	MobA_pBLO1
GenBank accession number	NP_862283
Family	MOB_Q
Length	565 aa
UniProt ID	Q8GN32
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.20E-65, Aligned region: 17..254
Note	putative relaxase
Protein sequence [Download]	MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGAP AEFADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEGYIRENLNADGYAA TYAIHDDGQGNNPHAHILVANRQIDPKTGGWAKLKQRMEYVLDERGERVPLIDPKTGAQK LDKRGRRQWKRTSVSLNPLDRKAKLQSLRESWAKTCNARLDETARIDHRSLEDQGSDLEP TIHEGYAARAIERAGGVSERCQTNREIRRSNDLLTAIRHELGRIFDRLGELFAAKIGQLR QRQARPEAAREPNWRYFEGDARRQLEADRADHAAALRGRLMDARADVRNRETWWKNHGTE EFEAAKQIIDVACEASREARDAGMFQRGRLRRNAEQVAGEQSERLRGAVPWLEDTEIPAD WDQANRFRMRTTKAIHDHDMQPRTGLVAELERQLEQAEQAAGERPSPEQVKDLALRMAEQ RERAEGQARGANRPEPSGDEPLDLSEPEDWGESSPFEQRATPKTKADLLGELKQRADRRT GRQEAEQRQIDPWSAQPPQRRGHGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	698
Plasmid name	pBLO1
GenBank accession number	NC_004943.1
Genome size	3626 bp
Coordinate of oriT [Strand]	3560..3591 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum NCC2705 [206672]

Reference

[1] Schell MA et al (2002) The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc Natl Acad Sci U S A. 99(22):14422-7. [PMID:12381787]