oriTDB

The information of the oriT region

oriTDB accession number	100235
Name	oriT_PNAC2
Sequence Completeness	core
oriT length	32 nt
oriT sequence	ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)	[1-8] [12-19] nt (ATGCAACC..GGTTGCAT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:1-8;R:12-19;D:28-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

Reference
N/A

I. Information of Relaxase

ID	306
Name	Mob_PNAC2
GenBank accession number	NP_848160
Family	MOB_Q
Length	565 aa
UniProt ID	Q83YJ4
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 1.20E-65, Aligned region: 17..254
Note	putative relaxase
Protein sequence [Download]	MAIYHLSVSNVSRASGSKATATLSYISGRRVHDERRGETYDYGRKERVLRVGTLLPEGAP AEFADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAA TYAIHEDREGNNPHAHILVANRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQK TDKRGRRQWKRTSVSLNPLDRKAKLKALRESWANTCNARLDETARIDHRSLEDQGSDLEP TIHEGYAARAIERAGGVSERCQTNREIRRSNSLLTAIRTELGRIFDRLGELFAAKIRQLR QRQARPEPAREPNWRYFEGDARRQLEADKADHTAAIRGKLMDARADVGNREEWWRSHGTE EHEAAKQIIDVARAASREARDANIFKRGRLLRNAEQVAGEQSEKLRGAVPWLEDTDIPAD WDQANRFRMRTTKAIYDHDMQPRTRLVAELERRLEQAERAESERPSPEQVKALALRMAEQ RAQAENPTQDTKRPAPSGDEPLDLSEPEDWGEASPFESRQAPRTKADLLNEIRQRTDRLT GQREAEQRQIDPWSAQPPQRRGHGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	697
Plasmid name	PNAC2
GenBank accession number	NC_004769.1
Genome size	3684 bp
Coordinate of oriT [Strand]	1082..1113 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum RW041 [216816]

Reference

[1] Corneau N et al (2004) Molecular characterization of three plasmids from Bifidobacterium longum. Plasmid. 51(2):87-100. [PMID:15003705]