oriTDB

The information of the oriT region

oriTDB accession number	100232
Name	oriT_pDOJH10L
Sequence Completeness	core
oriT length	32 nt
oriT sequence	ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)	[1-8] [12-19] nt (ATGCAACC..GGTTGCAT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:1-8;R:12-19;D:28-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

Reference
N/A

I. Information of Relaxase

ID	303
Name	Putative relaxase of pDOJH10L
GenBank accession number	WP_011067895
Family	MOB_Q
Length	370 aa
UniProt ID	Q8GN74
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.80E-54, Aligned region: 1..224
Note	putative relaxase
Protein sequence [Download]	MRDERTGEAFNGFGRRERVEHVCTMLPEGAPGEYLDPERLFNAVEMAEKRSDARPAKKIM VALPREFDARERFRALEDFISWNITANGYACTYAIHTDKDGRNPHAHILVANRRIDPKTG RWAAKEPQASSRDANGRRIPVIDPDTGRQKIGARNRKVWKRVNVSNNPLDSKEFLERLRR EWADSCNALLPGYAVIDHRSFKARGIERIPTIHEGYASREMEKRGGRGDLCEENRRIQAL NRLLDALRAMIGRLSDQAQGILTAVKRRQRPSGAPQAPSSPEPAERPQERPQARETPPQA PQEPPKPIRTKKALTDRFKTRLDERLAENERKQAEKQAEPDMEETWDLSDPADPLNIGMG WGTGGHGLGL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	694
Plasmid name	pDOJH10L
GenBank accession number	NC_004252.1
Genome size	10073 bp
Coordinate of oriT [Strand]	7357..7388 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum DJO10A [205913]

Reference

[1] Lee JH et al (2006) Sequence analysis of two cryptic plasmids from Bifidobacterium longum DJO10A and construction of a shuttle cloning vector. Appl Environ Microbiol. 72(1):527-35. [PMID:16391088]