oriTDB

The information of the oriT region

oriTDB accession number	100228
Name	oriT_pSFD10
Sequence Completeness	intact
oriT length	86 nt
oriT sequence	AGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGC TTAACCATGCGGCATCAGTGCAGATT
IR (inverted repeat)	_
Location of nic site	[59-60] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	D:59-60

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGC

61

TTAACCATGCGGCATCAGTGCAGATT

Reference
N/A

I. Information of Relaxase

ID	299
Name	MobA_pSFD10
GenBank accession number	NP_203138
Family	MOB_P
Length	517 aa
UniProt ID	Q93LC3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.70E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQYFTDGRGV REKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRRYSPVHNVDRGIAEKTPGRGE RGDDAAPEGRVKAGREYGRDVTGDSLSPVYREWRDALVSWREDTDEPGRNQGTGRDIAET EREDMGRGVCSGREKEISGPSVREISRGDALSGEGLDTTDGVIRNDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTALERADRTLEPVIQREL EIREERLIQEREHALSLERERQLEIQERTLDGPSLSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	690
Plasmid name	pSFD10
GenBank accession number	NC_003079.1
Incompatibility group	ColRNAI
Genome size	4091 bp
Coordinate of oriT [Strand]	991..1076 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica 79500 [28901]

Reference

[1] Liu M et al (2002) Sequence analysis and characterization of plasmid pSFD10 from Salmonella choleraesuis. Plasmid. 48(1):59-63. [PMID:12206756]