oriTDB

The information of the oriT region

oriTDB accession number	100227
Name	oriT_Ti
Sequence Completeness	core
oriT length	61 nt
oriT sequence	GGATCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGCGTAGGGGGCCAAACAGG G
IR (inverted repeat)	[19-27] [32-40] nt (ATACGTCGC..GCGACGCCT)
Location of nic site	[11-12] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:19-27;R:32-40;D:11-12

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGATCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGCGTAGGGGGCCAAACAGG

61

G

Reference
N/A

I. Information of Relaxase

ID	298
Name	TraA_Ti
GenBank accession number	NP_396650
Family	MOB_Q
Length	1100 aa
UniProt ID	Q44349
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 4.40E-76, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 1.10E-54, Aligned region: 381..569 Viral_helicase1 [PF01443.17], Evalue: 9.10E-05, Aligned region: 656..737 AAA_19 [PF13245.5], Evalue: 8.00E-09, Aligned region: 386..513 UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736
Note	putative relaxase
Protein sequence [Download]	MAIAHFSASIVSRGDGRSVVLSAAYQHCAKMEYEREARTIDYTRKQGLVHQEFILPADAP KWVRALIADCSVAGASEAFWNKVEAFEKRSDAQLARDLTIALPRELTSEQNIALVRDFVE KHILGKGMVADWVYHDNPGNPHIHLMTTLRPLTEDGFGAKKVAVIGEDGQLVRTKSGKIL YELWAGSTDDFNVVRDGWFERLNHHLTLGGIDLKIDGRSYEKQGIDLEPTIHLGVGAKAI SRKAEQQGVRPELERIELNEERRSENTRRILKNPAIVLDLIMREKSVFDERDVAKVLHRY VDDPAVFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEATMARQAMWLS DKETHAVSTAVLAATFGRHGRLSEEQKAAIECIAGPARIAAVVGRAGAGKTTMMKAAREA WELAGYRVVGGALAGKASEGLDKEAGIESRTLSSWELRWNRGRDVLDNKTVFVMDEAGMV ASKQMAGFVDAVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWM RKASLDLARGNVEKALALYNANARIVGERLKAEAVERLIADWNRDYDQTKTTLILAHLRR DVRMLNVMAREKLVERGIVGEGHVFRTADGERRFHAGDQIVFLKNETLLGVKNGMIGHVV EAVPNRIVAVVGDRDHRRHVVVEQRFYSNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDLQLYYGCRSFAFNGGLAKVLSRKNAKETTLDYERGKLYREALRFAENR GLHIMQVARTMLRDRLDWTLRQKTKVSDLVHRLRALGERLGLDQSPKTQTMKEAAPMVTG IKTFSGSVADTVGDKLGADPTLKQQWEEVSARFRYVFADPETAFRAVNFDTVLADKEAAK AVLQKLEAEPASIGALKGKTGILASKTEREARRVAEVNVPALKRDLEQYLRMRETATLRI VTEEQALRQRVSIDIPALSPAARVVLERVRDAIDRNDLPAALGYALSNRETKLEIDGFNQ AVTERFGERTLLSNVAREPSGKLYEKLSDGMRPEQKEQLKQAWPIMRTAQQLAAHERTVQ SLKQAEELRQTLRQAPVLKQ

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	689
Plasmid name	Ti
GenBank accession number	NC_003065.3
Genome size	214233 bp
Coordinate of oriT [Strand]	143868..143928 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	Most virulence determinants of A. tumefaciens have been found on the Ti plasmid.
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Agrobacterium fabrum str. C58 [176299]

Reference

[1] Goodner B et al (2001) Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science. 294(5550):2323-8. [PMID:11743194]

[2] Wood DW et al (2001) The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science. 294(5550):2317-23. [PMID:11743193]