oriTDB
The information of the oriT region
oriTDB accession number 100227
Name oriT_Ti  insolico
Sequence Completeness core
oriT length 61 nt
oriT sequence GGATCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGCGTAGGGGGCCAAACAGG G
IR (inverted repeat)[19-27] [32-40] nt  (ATACGTCGC..GCGACGCCT)
Location of nic site [11-12] nt
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L:19-27;R:32-40;D:11-12
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 298
Name TraA_Ti insolico
GenBank accession number NP_396650
Family MOBQ
Length 1100 aa
UniProt ID Q44349
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 4.40E-76, Aligned region: 17..243
AAA_30 [PF13604.5], Evalue: 1.10E-54, Aligned region: 381..569
Viral_helicase1 [PF01443.17], Evalue: 9.10E-05, Aligned region: 656..737
AAA_19 [PF13245.5], Evalue: 8.00E-09, Aligned region: 386..513
UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736
Note putative relaxase
Protein sequence [Download] MAIAHFSASIVSRGDGRSVVLSAAYQHCAKMEYEREARTIDYTRKQGLVHQEFILPADAP
KWVRALIADCSVAGASEAFWNKVEAFEKRSDAQLARDLTIALPRELTSEQNIALVRDFVE
KHILGKGMVADWVYHDNPGNPHIHLMTTLRPLTEDGFGAKKVAVIGEDGQLVRTKSGKIL
YELWAGSTDDFNVVRDGWFERLNHHLTLGGIDLKIDGRSYEKQGIDLEPTIHLGVGAKAI
SRKAEQQGVRPELERIELNEERRSENTRRILKNPAIVLDLIMREKSVFDERDVAKVLHRY
VDDPAVFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEATMARQAMWLS
DKETHAVSTAVLAATFGRHGRLSEEQKAAIECIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKASEGLDKEAGIESRTLSSWELRWNRGRDVLDNKTVFVMDEAGMV
ASKQMAGFVDAVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWM
RKASLDLARGNVEKALALYNANARIVGERLKAEAVERLIADWNRDYDQTKTTLILAHLRR
DVRMLNVMAREKLVERGIVGEGHVFRTADGERRFHAGDQIVFLKNETLLGVKNGMIGHVV
EAVPNRIVAVVGDRDHRRHVVVEQRFYSNLDHGYATTIHKSQGATVDRVKVLASLSLDRH
LTYVAMTRHREDLQLYYGCRSFAFNGGLAKVLSRKNAKETTLDYERGKLYREALRFAENR
GLHIMQVARTMLRDRLDWTLRQKTKVSDLVHRLRALGERLGLDQSPKTQTMKEAAPMVTG
IKTFSGSVADTVGDKLGADPTLKQQWEEVSARFRYVFADPETAFRAVNFDTVLADKEAAK
AVLQKLEAEPASIGALKGKTGILASKTEREARRVAEVNVPALKRDLEQYLRMRETATLRI
VTEEQALRQRVSIDIPALSPAARVVLERVRDAIDRNDLPAALGYALSNRETKLEIDGFNQ
AVTERFGERTLLSNVAREPSGKLYEKLSDGMRPEQKEQLKQAWPIMRTAQQLAAHERTVQ
SLKQAEELRQTLRQAPVLKQ
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 689
Plasmid name Ti
GenBank accession number NC_003065.3
Genome size 214233 bp
Coordinate of oriT  [Strand] 143868..143928 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico Most virulence determinants of A. tumefaciens have been found on the Ti plasmid.
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Agrobacterium fabrum str. C58 [176299]
Reference
[1] Goodner B et al (2001) Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science. 294(5550):2323-8. [PMID:11743194]
[2] Wood DW et al (2001) The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science. 294(5550):2317-23. [PMID:11743193]

Microbial Bioinformatics Group in MML, SJTU