oriTDB

The information of the oriT region

oriTDB accession number	100223
Name	oriT_pMD136
Sequence Completeness	incomplete
oriT length	63 nt
oriT sequence	GTAAATTGATTGATTAAGCTCGTGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATT TAC
IR (inverted repeat)	[1-31] [33-63] nt (GTAAATTGATTGATTAAGCTCGTGAAACCCT..AGGGTTTCACGAGCTTAATCAATCAATTTAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:1-31;R:33-63

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTAAATTGATTGATTAAGCTCGTGAAACCCTTAGGGTTTCACGAGCTTAATCAATCAATT

61

TAC

Reference
N/A

I. Information of Relaxase

ID	294
Name	Orf2_pMD136
GenBank accession number	NP_052581
Family	MOB_P
Length	386 aa
UniProt ID	Q9WW30
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.50E-64, Aligned region: 8..248
Note	putative relaxase
Protein sequence [Download]	MATTHIKRSDSASRLVNYAEKRAVLKDGLNLDVDYAKSQLKQVREVYGNQGKTQAYASRI SFSPKELDPTNENDQQKALEIAKEVYQKTYPNQQVALYEHADTNALHVHAVIGAIDLETG RKMHGNWQEYREKLVHNTDEISQEHGLEVTQVDPNRYEKRTMAEIKMQAKGQPTWKDQIR QAVDKTMANRLVTDFQTFRDDLKQKTVDVWERGKDLTYQLTGTNYKARGNKLGTAYEKGE IYHELERRTRNHTKTRNPDTAKPDRTQPNLERNQELSGHANSITEQTTDRIDRTAPTDRA SQSTGQSGFSNDLEQFRKQQRDQQTTINRDAQRRARTVQRANTQDKSKDNRSLKTDKQRP KEQQRDVEKSQQQSKSNRSRNDGPSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	685
Plasmid name	pMD136
GenBank accession number	NC_002126.1
Genome size	19533 bp
Coordinate of oriT [Strand]	19376..19438 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pediococcus pentosaceus ATCC 43200 [1255]

Reference

[1] Kantor A et al (1997) Molecular characterization of the replicon of the Pediococcus pentosaceus 43200 pediocin A plasmid pMD136. FEMS Microbiol Lett. 151(2):237-44. [PMID:9228759]