oriTDB

The information of the oriT region

oriTDB accession number	100222
Name	oriT_pUCD5000
Sequence Completeness	intact
oriT length	178 nt
oriT sequence	TTAGCCGGTTTCGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGG CTTCTATGTTAGTTCGAAGTGGTGATTCAGGAAAGTGCTTCATGTGACAGGGGGAAATGC AGCAAAAGTGCGTAGGGAGAAAATACAAGAACGGATGAAATGACGCCTCCTCGCTCAC
IR (inverted repeat)	[9-16] [20-27] nt (TTTCGGGC..GCCCTGAA)
Location of nic site	[60-61] nt
Conserved sequence flanking the nic site	CTGG\|CTTC
Note	predicted by the oriTfinder
	L:9-16;R:20-27;D:60-61

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGCCGGTTTCGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGG

61

CTTCTATGTTAGTTCGAAGTGGTGATTCAGGAAAGTGCTTCATGTGACAGGGGGAAATGC

121

AGCAAAAGTGCGTAGGGAGAAAATACAAGAACGGATGAAATGACGCCTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	293
Name	MobA_pUCD5000
GenBank accession number	NP_046807
Family	MOB_P
Length	421 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.90E-15, Aligned region: 1..204
Note	putative relaxase
Protein sequence [Download]	MASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRLQPYYDRADRPRID AWQTIVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQAITRGLLSLASSGELKTRQDV TEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAAEYRR DAESRIQRAREVCQNGTERKREENQRRHPRPRPEAVLSHESAYERDAAHGQPNVADHRPS IRAADSLERGHSVVAESADTRQLHDHPGAEEHGGHTLREEQRPATLDLWRAETPLREGEP GSGTVRRGFELDDTGGEIAHDRAGKTVAERIRAATAGLLEKAGRVGERLRGMADDVWSYA KGERGAERARHGLEQAGAEFERARTPVVVKLNSIEALREQHEKELELERSQRQQTYDGPS L

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	684
Plasmid name	pUCD5000
GenBank accession number	NC_001898.1
Genome size	5229 bp
Coordinate of oriT [Strand]	1385..1562 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Tatumella morbirosei 1056R [642227]

Reference

[1] Pujol CJ et al (1998) Characterization of pUCD5000 involved in pink disease color formation by Pantoea citrea. Plasmid. 40(2):169-73. [PMID:9735319]