oriTDB

The information of the oriT region

oriTDB accession number	100218
Name	oriT_R7K
Sequence Completeness	core
oriT length	39 nt
oriT sequence	GACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCC
IR (inverted repeat)	[9-14] [19-24] nt (CGCACC..GGTGCG)
Location of nic site	[32-33] nt
Conserved sequence flanking the nic site	TGTCT\|ATAGC
Note	_
	L:9-14;R:19-24;D:32-33

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCC

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]

I. Information of Relaxase

ID	211
Name	TrwC_R7K
GenBank accession number	YP_001874877
Family	MOB_F
Length	966 aa
UniProt ID	B2G2P0
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.20E-83, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 1.30E-45, Aligned region: 472..659 Viral_helicase1 [PF01443.17], Evalue: 2.50E-06, Aligned region: 492..697 UvrD_C_2 [PF13538.5], Evalue: 3.90E-07, Aligned region: 847..894 AAA_19 [PF13245.5], Evalue: 8.60E-06, Aligned region: 477..601
Note	conjugative relaxase
Protein sequence [Download]	MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL KNDEIVKATRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAARLEDIQ KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWIAELAAKGMKQGAARERVDNAI KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREA AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEA NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE AGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVELAAAGKASSSLERIKDVTEIKNHHER RAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAGKGIEFDTLVRVDTTQA ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQFSPMTH TKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGK LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAER GMEAGR

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	297	TrwA_R7K	YP_001874875	121 aa	mobilisation protein

Information of T4CP

ID	211
Name	TrwB_R7K
SecReT4 accesion number	187729595
GenBank accession number	YP_001874876
Family	TrwB/TraD
Length	507
UniProt ID	B2G2N9
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 1.30E-96, Aligned region: 109..491 TraG-D_C [PF12696.6], Evalue: 1.20E-20, Aligned region: 350..472 T4SS-DNA_transf [PF02534.13], Evalue: 3.40E-12, Aligned region: 297..489
Note	Type IV secretion-system coupling protein
Protein sequence [Download]	MHPDDQRKVSAGIVIVLPLIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGG LAVGVLFVWLLNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRD AEPRHLLVNGATGTGKSVLLRELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDVILNPYDK RTKGWSFFNEIRNDYDWQRYALSVVPRGKTDEAEEWASYGRLLLRETAKKLALIGTPSMR ELFHWTTIATFDDLRGFLEGTLAESLFAGSNEASKALTSARFVLSDKLPEHVTMPDGDFS IRSWLEDPNGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPKRRLWLFIDELAS LEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPVEIANLPDLTAYVGFA GNRPIAKVPLEIKQFANRQPAFVEGTI

Reference
N/A

Information of plasmid

ID	211
Plasmid name	R7K
GenBank accession number	NC_010643.1
Incompatibility group	IncW
Genome size	39792 bp
Coordinate of oriT [Strand]	9136..9174 [-]
Drug resistance	resistance to ampicillin, streptomycin and spectinomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Providencia rettgeri [587]

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]