 oriTDB accession number | 100218 |
| Name | oriT_R7K  |
Sequence Completeness  | core |
| oriT length | 39 nt |
| oriT sequence | GACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCC |
| IR (inverted repeat) | [9-14] [19-24] nt (CGCACC..GGTGCG) |
| Location of nic site | [32-33] nt |
| Conserved sequence flanking the nic site | TGTCT|ATAGC |
| Note | _ |
Visualization of oriT structure Reference| [1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088] |
| ID | 211 |
| Name | TrwC_R7K  |
| GenBank accession number | YP_001874877 |
| Family | MOBF |
| Length | 966 aa |
| UniProt ID | B2G2P0 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 1.20E-83, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 1.30E-45, Aligned region: 472..659 Viral_helicase1 [PF01443.17], Evalue: 2.50E-06, Aligned region: 492..697 UvrD_C_2 [PF13538.5], Evalue: 3.90E-07, Aligned region: 847..894 AAA_19 [PF13245.5], Evalue: 8.60E-06, Aligned region: 477..601 |
| Note | conjugative relaxase |
| Protein sequence [Download] | MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL KNDEIVKATRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAARLEDIQ KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWIAELAAKGMKQGAARERVDNAI KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREA AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEA NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE AGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVELAAAGKASSSLERIKDVTEIKNHHER RAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAGKGIEFDTLVRVDTTQA ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQFSPMTH TKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGK LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAER GMEAGR |
Reference| [1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 297 | TrwA_R7K | YP_001874875 | 121 aa | mobilisation protein |
| ID | 211 |
| Name | TrwB_R7K |
| SecReT4 accesion number | 187729595 |
| GenBank accession number | YP_001874876 |
| Family | TrwB/TraD |
| Length | 507 |
| UniProt ID | B2G2N9 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 1.30E-96, Aligned region: 109..491 TraG-D_C [PF12696.6], Evalue: 1.20E-20, Aligned region: 350..472 T4SS-DNA_transf [PF02534.13], Evalue: 3.40E-12, Aligned region: 297..489 |
| Note | Type IV secretion-system coupling protein |
| Protein sequence [Download] | MHPDDQRKVSAGIVIVLPLIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGG LAVGVLFVWLLNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRD AEPRHLLVNGATGTGKSVLLRELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDVILNPYDK RTKGWSFFNEIRNDYDWQRYALSVVPRGKTDEAEEWASYGRLLLRETAKKLALIGTPSMR ELFHWTTIATFDDLRGFLEGTLAESLFAGSNEASKALTSARFVLSDKLPEHVTMPDGDFS IRSWLEDPNGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPKRRLWLFIDELAS LEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPVEIANLPDLTAYVGFA GNRPIAKVPLEIKQFANRQPAFVEGTI |
Reference| ID | 211 |
| Plasmid name | R7K |
| GenBank accession number | NC_010643.1 |
| Incompatibility group | IncW |
| Genome size | 39792 bp |
| Coordinate of oriT [Strand] | 9136..9174 [-] |
| Drug resistance | resistance to ampicillin, streptomycin and spectinomycin |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Providencia rettgeri [587] |
Reference| [1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088] |