oriTDB

The information of the oriT region

oriTDB accession number	100217
Name	oriT_pIE1107
Sequence Completeness	core
oriT length	37 nt
oriT sequence	CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC
IR (inverted repeat)	[3-8] [14-19] nt (GTTTCTT..AGAAAC)
Location of nic site	[30-31] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	similar to the oriT of IncQ plasmid RSF101
	L:3-8;R:14-19;D:30-31

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]

[2] Tietze E (1998) Nucleotide sequence and genetic characterization of the novel IncQ-like plasmid pIE1107. Plasmid. 39(3):165-81. [PMID:9571133]

N/A

Information of plasmid

ID	210
Plasmid name	pIE1107
GenBank accession number	Z74787.1
Incompatibility group	IncQ-like
Genome size	8520 bp
Coordinate of oriT [Strand]	943..979 [-]
Drug resistance	resistance to streptothricin and kanamycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]