oriTDB
The information of the oriT region
oriTDB accession number 100215
Name oriT_pIE1115  insolico
Sequence Completeness core
oriT length 37 nt
oriT sequence CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC
IR (inverted repeat)[3-8] [14-19] nt  (GTTTCTT..AGAAAC)
Location of nic site [30-31] nt
Conserved sequence flanking the
  nic site 		TAAGTGCG|CCCT
Note similar to the oriT of IncQ plasmid RSF101
L:3-8;R:14-19;D:30-31
Visualization of oriT structure
Reference
[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]
I. Information of Relaxase
ID 208
Name MobA_pIE1115 insolico
GenBank accession number NP_065281
Family MOBQ
Length 703 aa
UniProt ID Q9FBF9
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 9.50E-47, Aligned region: 394..641
MobA_MobL [PF03389.14], Evalue: 4.00E-34, Aligned region: 16..218
Note relaxase
Protein sequence [Download] MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDG
ADLYERANGRLFKEVEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPH
CHLMISERKNDGIERPADQWFKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRA
LEQAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMESRGIRTERADIALAIDTANGQIID
LQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTGGRSPAADRRGTAGQRGAGRE
LDEPATASHERMAAGSKGHEGSRRGTGQGRPDGPERRPWLDMEVVGRGLDRFRDAYSGAA
DRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM
NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSIDDLDAMKQEGREPA
AIIETSPKNYQAWVKVAQDAPAGHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDK
YTSRTGYQPWVLCRESSGKSATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRY
RRSVVDDYRSEMAGLVKRYGDDLSKCDFIAAMKLASKGREPDEIGKAMAEASPAIMERKA
GHEADYIKRTVQKVMELPQVQEARAELAKQAQKQRSRGPDLSM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 292 MobB_pIE1115 insolico NP_065282 136 aa putative role in conjugative DNA replication
2 293 MobC_pIE1115 insolico NP_065280 95 aa putative role in conjugative DNA replication
 N/A
Information of plasmid
ID 208
Plasmid name pIE1115
GenBank accession number NC_002524.1
Incompatibility group IncQ-like
Genome size 10687 bp
Coordinate of oriT  [Strand] 371..407 [-]
Drug resistance experimental resistance to and sulfonamide
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]

Microbial Bioinformatics Group in MML, SJTU