oriTDB

The information of the oriT region

oriTDB accession number	100215
Name	oriT_pIE1115
Sequence Completeness	core
oriT length	37 nt
oriT sequence	CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC
IR (inverted repeat)	[3-8] [14-19] nt (GTTTCTT..AGAAAC)
Location of nic site	[30-31] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	similar to the oriT of IncQ plasmid RSF101
	L:3-8;R:14-19;D:30-31

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CAGTTTCTTGAAGAGAAACCGGTAAGTGCGCCCTCCC

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]

I. Information of Relaxase

ID	208
Name	MobA_pIE1115
GenBank accession number	NP_065281
Family	MOB_Q
Length	703 aa
UniProt ID	Q9FBF9
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 9.50E-47, Aligned region: 394..641 MobA_MobL [PF03389.14], Evalue: 4.00E-34, Aligned region: 16..218
Note	relaxase
Protein sequence [Download]	MAIYHLTAKTGSRNGGQSAKAKADYIQREGRYSRDREEVLHTQSGHLPEWAERPADYWDG ADLYERANGRLFKEVEFALPVELTLDQQRELVDEFARHLTEGERLPYTLAIHAGNGENPH CHLMISERKNDGIERPADQWFKRYNGKQPERGGAQKSESLKPKAWLEQTREAWSHYANRA LEQAGHEARIDHRTLEAQGIERLPGIHLGPNVVEMESRGIRTERADIALAIDTANGQIID LQEYREVIEHERDRQSEEIQRDQRVSGRDRAAGPEHGDTGGRSPAADRRGTAGQRGAGRE LDEPATASHERMAAGSKGHEGSRRGTGQGRPDGPERRPWLDMEVVGRGLDRFRDAYSGAA DRIMALAGAANHRATGEHMADFQAQVKGDRTAQAIARQLKAMGCDRYDIGIRDAASGKMM NREWTPQEVQQNAAWLKRMNAQGNDIYIRPAEQARHGLVLVDDLSIDDLDAMKQEGREPA AIIETSPKNYQAWVKVAQDAPAGHRGVIARKLAREYDADPASADSRHYGRLAGFTNRKDK YTSRTGYQPWVLCRESSGKSATAGPELMQQAGQVLDSIERRQERTARLAEITAPQSVRRY RRSVVDDYRSEMAGLVKRYGDDLSKCDFIAAMKLASKGREPDEIGKAMAEASPAIMERKA GHEADYIKRTVQKVMELPQVQEARAELAKQAQKQRSRGPDLSM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	292	MobB_pIE1115	NP_065282	136 aa	putative role in conjugative DNA replication
2	293	MobC_pIE1115	NP_065280	95 aa	putative role in conjugative DNA replication

N/A

Information of plasmid

ID	208
Plasmid name	pIE1115
GenBank accession number	NC_002524.1
Incompatibility group	IncQ-like
Genome size	10687 bp
Coordinate of oriT [Strand]	371..407 [-]
Drug resistance	resistance to and sulfonamide
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]