oriTDB

The information of the oriT region

oriTDB accession number	100214
Name	oriT_pLS55
Sequence Completeness	incomplete
oriT length	35 nt
oriT sequence	CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[6-9] [10-13] nt (AGAA..TTCT)
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:6-9;R:10-13;D:24-25

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

I. Information of Relaxase

ID	207
Name	Mob_pLS55
GenBank accession number	YP_001691307
Family	MOB_V
Length	439 aa
UniProt ID	B0YMX1
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note	relaxase
Protein sequence [Download]	MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL SQQRQQQKKTKSRSSGMEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	291	_

N/A

Information of plasmid

ID	207
Plasmid name	pLS55
GenBank accession number	NC_010375.1
Genome size	5031 bp
Coordinate of oriT [Strand]	2038..2072 [+]
Drug resistance	resistance to tetracycline (tetL)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus sakei Rits9 [1599]

Reference
N/A