 oriTDB accession number | 100213 |
| Name | oriT_pAKD33  |
Sequence Completeness  | intact |
| oriT length | 304 nt |
| oriT sequence | TGCCTCGCAGAGCAGGATGACCCGTTGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGA TAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTGCCCGCCTTACGGCGTTAAT AACACCAAGGAAAGTCTACACCAGCCATTACGATTTATCCGCAACTATCGCGCTATCAGG CCGCAAAAGCAGCAACGGATATAGCGAAAACCGCCACAATGGCCCATAATGCCGCTATCG AAGCGTGCCAATGCACGCCGATAGCGGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGC GTTA |
| IR (inverted repeat) | [55-72] [75-92] nt (TGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCA) |
| Location of nic site | [101-102] nt |
| Conserved sequence flanking the nic site | CATCCTG|C |
| Note | similar to oriT of the IncP-1beta multiresistance plasmid pB10 |
Visualization of oriT structure Reference| [1] Sen D et al (2011) Broad-host-range plasmids from agricultural soils have IncP-1 backbones with diverse accessory genes. Appl Environ Microbiol. 77(22):7975-83. [PMID:21948829] |
| ID | 206 |
| Name | TraI_pAKD33 |
| GenBank accession number | AEV57343 |
| Family | MOBP |
| Length | 746 aa |
| UniProt ID | Q7X3D1 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252 |
| Note | conjugal transfer relaxase TraI; Provisional |
| Protein sequence [Download] | MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSELQAINKQFQQ ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR LTRIAVGDAVSITAKGSIKTSKGRSR |
Reference| # | ID | Name | GenBank | Length | Note |
| 1 | 288 | TraH_pAKD33 | AEV57344 | 130 aa | DNA-processing protein; relaxosome stabilisation |
| 2 | 289 | TraJ_pAKD33 | AEV57345 | 124 aa | conjugal transfer relaxosome component TraJ |
| 3 | 290 | TraK_pAKD33 | AEV57346 | 132 aa | DNA-processing protein; oriT-binding protein |
| ID | 206 |
| Name | TraG_pAKD33 |
| SecReT4 accesion number | _ |
| GenBank accession number | AEV57342 |
| Family | VirD4/TraG |
| Length | 637 |
| UniProt ID | Q7X3D2 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586 |
| Note | conjugal transfer coupling protein TraG |
| Protein sequence [Download] | MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL YFKDPVFSARAAIPAPKVSDRLRAVAQAETEGEGITI |
Reference| ID | 206 |
| Plasmid name | pAKD33 |
| GenBank accession number | JN106174.1 |
| Incompatibility group | IncP1 |
| Genome size | 57974 bp |
| Coordinate of oriT [Strand] | 29904..30207 [+] |
| Drug resistance | _ |
| Heavy-metal resistance |  mercury resistance |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Uncultured bacterium [77133] |
Reference| [1] Sen D et al (2011) Broad-host-range plasmids from agricultural soils have IncP-1 backbones with diverse accessory genes. Appl Environ Microbiol. 77(22):7975-83. [PMID:21948829] |