oriTDB

The information of the oriT region

oriTDB accession number	100212
Name	oriT_R27
Sequence Completeness	intact
oriT length	285 nt
oriT sequence	GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCAC ATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGT TACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAAT TCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTA CAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT
IR (inverted repeat)	[189-194] [196-201] nt (AAAAGT..ACTTTT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:189-194;R:196-201

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCAC

61

ATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGT

121

TACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAAT

181

TCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTA

241

CAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Reference

[1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349]

I. Information of Relaxase

ID	205
Name	TraI_R27
GenBank accession number	NP_058333
Family	MOB_H
Length	1011 aa
UniProt ID	Q9L5M2
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 4.30E-35, Aligned region: 99..398
Note	-
Protein sequence [Download]	MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKF IEYPTRPAGIQTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIR WIHLLPASENHHHNGIGGLLSHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAF ICGLVHDAGKVYDLDIVSLNLSETLTWAPSSQSLLDWARENNVVEYEIHWRKRIHNQHNI WSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDGNDFLSKCVRTSDYYSTGTDL NVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVYLNENAFLDFV LSDFAAHKFNFPQGDAGKTVLVESLVQRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRE SVTRVTDTVNDAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFE TQVVTAIDTAETVNADEPEQVEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLD LDAAIALIPGIDEAAFCNGPFFQLTYRDGSLDGMWIVRDVNNLRLIQLGDNCAGMQVSTS EPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLELPQPRLNAPVAEEASSVAEQ TNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATDPQSSDFAQLP RETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDG EVTELEYVEIATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPDL FNQLPQYQVTSGAVVNTKCIAFNIPTLVAASDRAKVDIELIINKLKEVGNL

Reference

[1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349]

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

Information of T4CP

ID	205
Name	TraG_R27
SecReT4 accesion number	_
GenBank accession number	NP_058332
Family	TrwB/TraD
Length	694
UniProt ID	Q9L5M3
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 8.50E-10, Aligned region: 396..648 TraG-D_C [PF12696.6], Evalue: 1.30E-08, Aligned region: 504..632 AAA_10 [PF12846.6], Evalue: 1.20E-06, Aligned region: 194..304
Note	_
Protein sequence [Download]	MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLL VCLYRCKTAHPYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKTNRP VPDRKTYFCNGFEWGPEHADRAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNA IFAVDRRAPIFVTEDNWFGHTLITGNVGTGKTVLQRLLSISMLHLGHVIVVIDPKNDAEW RQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSDLTSRLLSLVSVPGEVNPFVQ YAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARHYGPDVWMEKV KYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLI MSDLTSCAGSRYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQT ISDFIAAANAETANRITGLCNNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETT LPHNNFSGSISERKQTTLEESIPKELLGQVPKFHIVARLQDGRKVVGQIPIAVSEKAMKP NTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

Reference

[1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349]

Information of plasmid

ID	205
Plasmid name	R27
GenBank accession number	NC_002305.1
Incompatibility group	IncHI1
Genome size	180461 bp
Coordinate of oriT [Strand]	108637..108921 [+]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhi (Salmonella typhi) [90370]

Reference

[1] Lawley TD et al (2002) Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27. J Bacteriol. 184(8):2173-80. [PMID:11914349]