oriTDB

The information of the oriT region

oriTDB accession number	100211
Name	oriT_pRS01
Sequence Completeness	core
oriT length	40 nt
oriT sequence	TCCGTAAGATGCTATCATCTTACTATGCTTGCAAAAGGTC
IR (inverted repeat)	[4-11] [16-23] nt (GTAAGATG..CATCTTAC)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	_
	L:4-11;R:16-23;D:31-32

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCGTAAGATGCTATCATCTTACTATGCTTGCAAAAGGTC

Reference

[1] Novikova O et al (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]

I. Information of Relaxase

ID	204
Name	LtrB_pRS01
GenBank accession number	AAB06502
Family	MOB_P
Length	563 aa
UniProt ID	Q48722
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.50E-82, Aligned region: 34..307
Note	relaxase
Protein sequence [Download]	MVYTKHIIVHKLKHLRQAKDYVENAEKTLVNESNEDHLTNLFPYISNPDKTMSKQLVSGH GITNVYDAANEFIATKKLKALSKGTDFNFDPQTGKVRFNVESLEKNNAVLGHHLIQSFSP DDNLTPEQIHEIGRQTILEFTGGEYEFVIATHVDREHIHNHIIFNSTNLYTGKQFDWKVI PKEKTKSGKAYDVTKNNFEKVSDKIASRYGAKIIEKSPGNSHLKYTKWQTQSIYKSQIKQ RLDYLLEMSSDIEDFKRKATALNLSFDFSGKWTTYRLLDEPQMKNTRGRNLDKNRPEKYN LESIIERLETNELSLTVDEVVERYEEKVDVVKQDFDYQVTVEKGQIDHMTSKGFYLNVDF GIADRGQIFIGGYKVDQLENRDCVLYLKKNETFRLLSEKEASFTKYLTGHDLAKQLGLYN GTVPLKKEPVISTINQLVDAINFLAEHGVTEGTQFNNMESQLMSALGEAEEKLYVIDNKI MELTKIAKLLIEKESDHSQAVINELENLGVGPSIKYQDIHQELQSEKMSRKILKNKFEQT VDEINTFNEIRVTTLEENKGKIL

Reference

[1] Novikova O et al (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]

[2] Mills DA et al (1996) Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci. J Bacteriol. 178(12):3531-8. [PMID:8655550]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	286	_

N/A

Information of plasmid

ID	204
Plasmid name	pRS01
GenBank accession number	U50902.4
Genome size	48 kb
Coordinate of oriT [Strand]	5497..5536 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis ML3 [1360]

Reference
N/A