oriTDB
The information of the oriT region
oriTDB accession number 100211
Name oriT_pRS01  experimental
Sequence Completeness core
oriT length 40 nt
oriT sequence TCCGTAAGATGCTATCATCTTACTATGCTTGCAAAAGGTC
IR (inverted repeat)[4-11] [16-23] nt  (GTAAGATG..CATCTTAC)
Location of nic site [31-32] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note _
L:4-11;R:16-23;D:31-32
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Novikova O et al (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]
I. Information of Relaxase
ID 204
Name LtrB_pRS01 experimental
GenBank accession number AAB06502
Family MOBP
Length 563 aa
UniProt ID Q48722
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.50E-82, Aligned region: 34..307
Note relaxase
Protein sequence [Download] MVYTKHIIVHKLKHLRQAKDYVENAEKTLVNESNEDHLTNLFPYISNPDKTMSKQLVSGH
GITNVYDAANEFIATKKLKALSKGTDFNFDPQTGKVRFNVESLEKNNAVLGHHLIQSFSP
DDNLTPEQIHEIGRQTILEFTGGEYEFVIATHVDREHIHNHIIFNSTNLYTGKQFDWKVI
PKEKTKSGKAYDVTKNNFEKVSDKIASRYGAKIIEKSPGNSHLKYTKWQTQSIYKSQIKQ
RLDYLLEMSSDIEDFKRKATALNLSFDFSGKWTTYRLLDEPQMKNTRGRNLDKNRPEKYN
LESIIERLETNELSLTVDEVVERYEEKVDVVKQDFDYQVTVEKGQIDHMTSKGFYLNVDF
GIADRGQIFIGGYKVDQLENRDCVLYLKKNETFRLLSEKEASFTKYLTGHDLAKQLGLYN
GTVPLKKEPVISTINQLVDAINFLAEHGVTEGTQFNNMESQLMSALGEAEEKLYVIDNKI
MELTKIAKLLIEKESDHSQAVINELENLGVGPSIKYQDIHQELQSEKMSRKILKNKFEQT
VDEINTFNEIRVTTLEENKGKIL
Reference
[1] Novikova O et al (2014) Interaction between conjugative and retrotransposable elements in horizontal gene transfer. PLoS Genet. 10(12):e1004853. [PMID:25474706]
[2] Mills DA et al (1996) Splicing of a group II intron involved in the conjugative transfer of pRS01 in lactococci. J Bacteriol. 178(12):3531-8. [PMID:8655550]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 286 _ insolico
 N/A
Information of plasmid
ID 204
Plasmid name pRS01
GenBank accession number U50902.4
Genome size 48 kb
Coordinate of oriT  [Strand] 5497..5536 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis ML3 [1360]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU