oriTDB
The information of the oriT region
oriTDB accession number 100210
Name oriT_pSB102  insolico
Sequence Completeness incomplete
oriT length 42 nt
oriT sequence CTTTACCGGCGTTAGCCTATCCTGCAATAGACCTCACAAGCC
IR (inverted repeat)_
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note
D:24-25
Visualization of oriT structure
Reference
[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]
I. Information of Relaxase
ID 203
Name TraS_pSB102 insolico
GenBank accession number CAC79161
Family MOBP
Length 352 aa
UniProt ID Q91US6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 3.00E-15, Aligned region: 71..296
Note relaxase
Protein sequence [Download] MAKREIDGVLKDWGERVDYGRVQGRKGKNIAGGRYSKPAPEKKPSGREKLEATVRKAPEV
MVKISGGGKDMRSIKAHMDYISRNGAVELEDEQGRVHQGKEDVRAVRDDWRGGGIPYEDG
TKREAFNIVLSMPPGTNRQAVKDAARAFAAELFGNHQYVFAAHDDEKHPHVHLAVKAVDL
DGVRLNPRKADLQNWRETFAEKLRAQGIDANATPRKARGIVRKAEKQAVVHIEQRGKTPR
VKASRRADAEREASGVAVKPNPAQDRISVTRKDVQRAYGTAARALAKGEAKDKQLAVEIV
HFVQQMPPPITQHEAMVRQLREKKRDDARTIGPAKTKETQQDKDQGGPDQSR
Reference
[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 285 _ insolico
Information of T4CP
ID 203
Name TraN/VirD4_pSB102 insolico
SecReT4 accesion number 15919977
GenBank accession number CAC79168
Family VirD4/TraG
Length 909
UniProt ID Q91UR9
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 3.40E-34, Aligned region: 94..298
T4SS-DNA_transf [PF02534.13], Evalue: 8.20E-59, Aligned region: 382..651
TraG-D_C [PF12696.6], Evalue: 1.80E-20, Aligned region: 482..608
TrwB_AAD_bind [PF10412.8], Evalue: 4.00E-09, Aligned region: 385..624
Note Type IV secretory pathway, VirD4 component,TraG/TraD family ATPase
Protein sequence [Download] MEIPKWLKWLLGIVGFIAATAGAIWLSGFLFFAFSKTNPMGKTDFWTWWTYWQFYAADPV
VAKRLKVALIVAAVVAYGVPLVAIIAAMREVRSLHGDARFATAGEIAKAGLFAKTGIIVG
KWGRRYLMYASMQFVLLAAPTRSGKGVGIVIPNLLNWAESVVVLDVKLENFLITSKYRAK
WGQEVFLFNPFSISEDADGNPLHGKTHRYNPLGYVSDDPRLRVTDILAIGYSLYPGEGRD
AFFDDAARNLFLGLTLYLCETPTLPRTMGELLRQSSGKGRPVKEHIQGFITERNYREYGD
LMLNDLGDDREAVVQAVMAIRGCDEAEAEALCDDVPVAVVENVRNTELDQFELLLSGAGA
EFESDRRLVPLTVWDGEGLPMLSMECVDALNRFTSTSDNTLSSIMATLHVPLTIWASPIV
DAATSANDFDLRDVRKKRMSIYLGIPANKLSEAKLLLNMFYTQLVNLNTNQLLHSTPELK
FTCLMLDDEFTAPGRIGIIDKANSYMAGYGLRLLTIIQSPGQLEAESPKGYGRESARTFV
TNHALQILYTPKEQRDANEYSEMLGYMTVHSRNRSHGKSGSSVSEAEGEGAGQKRALMMP
QELKEMSQRKQIISLENMKPIKCDKISYFSDHAFMDRLKSVSPTLAALGKKLPSKKQLED
TWGSGELAAPVPDLNLDLHEAVVQSRVREMTVADVDKGIDLRALAVDTSKIAVVAGSDGL
EPDEIEGFVNDFFDALDAADPNEYDDDAPVDDDGSGALPSDDELAALDAEAEAEALAETN
ANTAAEEVPQAAPAAPKPEPKPKAKRKPKAAPAVAPVVAVAAVAEEVAVQADENLRHDEG
ELVADEPTDDELAAMMDDVFPDDGHLPDEADMLAALDEMEDVPDLPPELEDDAPILDLSV
LDKPVSQNR

Reference
[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]
Information of plasmid
ID 203
Plasmid name pSB102
GenBank accession number AJ304453.1
Incompatibility group PromA
Genome size 55578 bp
Coordinate of oriT  [Strand] 11400..11441 [-]
Drug resistance _
Heavy-metal resistance experimental mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]

Microbial Bioinformatics Group in MML, SJTU