oriTDB

The information of the oriT region

oriTDB accession number	100210
Name	oriT_pSB102
Sequence Completeness	incomplete
oriT length	42 nt
oriT sequence	CTTTACCGGCGTTAGCCTATCCTGCAATAGACCTCACAAGCC
IR (inverted repeat)	_
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note
	D:24-25

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTTACCGGCGTTAGCCTATCCTGCAATAGACCTCACAAGCC

Reference

[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]

I. Information of Relaxase

ID	203
Name	TraS_pSB102
GenBank accession number	CAC79161
Family	MOB_P
Length	352 aa
UniProt ID	Q91US6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.00E-15, Aligned region: 71..296
Note	relaxase
Protein sequence [Download]	MAKREIDGVLKDWGERVDYGRVQGRKGKNIAGGRYSKPAPEKKPSGREKLEATVRKAPEV MVKISGGGKDMRSIKAHMDYISRNGAVELEDEQGRVHQGKEDVRAVRDDWRGGGIPYEDG TKREAFNIVLSMPPGTNRQAVKDAARAFAAELFGNHQYVFAAHDDEKHPHVHLAVKAVDL DGVRLNPRKADLQNWRETFAEKLRAQGIDANATPRKARGIVRKAEKQAVVHIEQRGKTPR VKASRRADAEREASGVAVKPNPAQDRISVTRKDVQRAYGTAARALAKGEAKDKQLAVEIV HFVQQMPPPITQHEAMVRQLREKKRDDARTIGPAKTKETQQDKDQGGPDQSR

Reference

[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	285	_

Information of T4CP

ID	203
Name	TraN/VirD4_pSB102
SecReT4 accesion number	15919977
GenBank accession number	CAC79168
Family	VirD4/TraG
Length	909
UniProt ID	Q91UR9
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 3.40E-34, Aligned region: 94..298 T4SS-DNA_transf [PF02534.13], Evalue: 8.20E-59, Aligned region: 382..651 TraG-D_C [PF12696.6], Evalue: 1.80E-20, Aligned region: 482..608 TrwB_AAD_bind [PF10412.8], Evalue: 4.00E-09, Aligned region: 385..624
Note	Type IV secretory pathway, VirD4 component,TraG/TraD family ATPase
Protein sequence [Download]	MEIPKWLKWLLGIVGFIAATAGAIWLSGFLFFAFSKTNPMGKTDFWTWWTYWQFYAADPV VAKRLKVALIVAAVVAYGVPLVAIIAAMREVRSLHGDARFATAGEIAKAGLFAKTGIIVG KWGRRYLMYASMQFVLLAAPTRSGKGVGIVIPNLLNWAESVVVLDVKLENFLITSKYRAK WGQEVFLFNPFSISEDADGNPLHGKTHRYNPLGYVSDDPRLRVTDILAIGYSLYPGEGRD AFFDDAARNLFLGLTLYLCETPTLPRTMGELLRQSSGKGRPVKEHIQGFITERNYREYGD LMLNDLGDDREAVVQAVMAIRGCDEAEAEALCDDVPVAVVENVRNTELDQFELLLSGAGA EFESDRRLVPLTVWDGEGLPMLSMECVDALNRFTSTSDNTLSSIMATLHVPLTIWASPIV DAATSANDFDLRDVRKKRMSIYLGIPANKLSEAKLLLNMFYTQLVNLNTNQLLHSTPELK FTCLMLDDEFTAPGRIGIIDKANSYMAGYGLRLLTIIQSPGQLEAESPKGYGRESARTFV TNHALQILYTPKEQRDANEYSEMLGYMTVHSRNRSHGKSGSSVSEAEGEGAGQKRALMMP QELKEMSQRKQIISLENMKPIKCDKISYFSDHAFMDRLKSVSPTLAALGKKLPSKKQLED TWGSGELAAPVPDLNLDLHEAVVQSRVREMTVADVDKGIDLRALAVDTSKIAVVAGSDGL EPDEIEGFVNDFFDALDAADPNEYDDDAPVDDDGSGALPSDDELAALDAEAEAEALAETN ANTAAEEVPQAAPAAPKPEPKPKAKRKPKAAPAVAPVVAVAAVAEEVAVQADENLRHDEG ELVADEPTDDELAAMMDDVFPDDGHLPDEADMLAALDEMEDVPDLPPELEDDAPILDLSV LDKPVSQNR

Reference

[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]

Information of plasmid

ID	203
Plasmid name	pSB102
GenBank accession number	AJ304453.1
Incompatibility group	PromA
Genome size	55578 bp
Coordinate of oriT [Strand]	11400..11441 [-]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Schneiker S et al (2001) The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res. 29(24):5169-81. [PMID:11812851]