oriTDB

The information of the oriT region

oriTDB accession number	100208
Name	oriT_pAM373
Sequence Completeness	incomplete
oriT length	13 nt
oriT sequence	TAAAATGGTCAGG
IR (inverted repeat)	_
Location of nic site	[6-7] nt
Conserved sequence flanking the nic site	_
Note	CloDF13 familiy
	D:6-7

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TAAAATGGTCAGG

Reference

[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

[2] Francia MV et al (2002) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. Mol Microbiol. 45(2):375-95. [PMID:12123451]

N/A

Information of T4CP

ID	201
Name	TraG_pAM373
SecReT4 accesion number	_
GenBank accession number	NP_072029
Family	_
Length	423
UniProt ID	Q9F1G0
PDB ID	_
Pfam	CHAP [PF05257.15], Evalue: 8.20E-18, Aligned region: 299..393 Glucosaminidase [PF01832.19], Evalue: 1.30E-06, Aligned region: 68..216
Note	traG-related protein
Protein sequence [Download]	MNKKRIVFGLLSFFLPIFLVFGGLLFFLLLLTSTSDTSKNDCIQPSINNPTDATDTPKSI EQFVKSHKDAYLLSWKAGGFLPSASISQTMVENGFNFTNPSGTSFWQAHNMGGVKTSKKE DFPVTLATFGQDSVDISGTKPGSNVGDGTGGAYTWFKDYNAGIVGKAEFMAHQTLYTGAI NNTDGLSTLSAIYSGGWATDPTYLMKLQATYNSLGKQFQWLDQEAIQKYGNAPFKKSELV PNIPGKSPITNEKSGKNSDCVVTSDTSDQVTGQNTAPSLEVPSAYKGKLTLPPIDSNDYA GNNYPFGQCTWYAYNRMAQIGKPIEWFSGDGGNGAGWANSARAKGYTVVKGKPSVGWAAS MQGGIGGSAPPYGHVAVVEYVNSDGSILVSEANVINQGSGTRSWRVLDRATVEQIDFIQG KGA

Reference

[1] Francia MV et al (2004) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

[2] Francia MV et al (2002) Transfer origins in the conjugative Enterococcus faecalis plasmids pAD1 and pAM373: identification of the pAD1 nic site, a specific relaxase and a possible TraG-like protein. Mol Microbiol. 45(2):375-95. [PMID:12123451]

Information of plasmid

ID	201
Plasmid name	pAM373
GenBank accession number	NC_002630.1
Genome size	36804 bp
Coordinate of oriT [Strand]	14431..14443 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecalis [1351]

Reference
N/A