oriTDB
The information of the oriT region
oriTDB accession number 100203
Name oriT_pJHCMW1  insolico
Sequence Completeness intact
oriT length 218 nt
oriT sequence GATGGCATGGGGTGATATCCAGTAGCTAAGGGGTTGTTAGCGGGTTTCGGGGCGCAGCCC TGAATCAGTCATGTAACACTAGCAGAGTGTACACGGGCTAAATATGTTACGGAGGAGTAA CACTTCGCGAAAGTGCTTCATATGGCAGAGGGAAAATGCAGCGCTAGAGCGTACAGGAGA AAATAGAGGTTACGGTGATATGACGCTTCTTCGCTCAT
IR (inverted repeat)[46-53] [57-64] nt  (TTCGGGGC..GCCCTGAA)
[76-80] [86-90] nt  (ACACT..AGTGT)
[106-110] [117-122] nt  (GTTAC..GTAACA)
Location of nic site [97-98] nt
Conserved sequence flanking the
  nic site
CGGG|CTAA
Note _
L:46-53;R:57-64;L:76-80;RL86-90;L:106-110;R:117-122;D:97-98
Visualization of oriT structure
Reference
[1] Dery KJ et al (1997) Characterization of the replication and mobilization regions of the multiresistance Klebsiella pneumoniae plasmid pJHCMW1. Plasmid. 38(2):97-105. [PMID:9339467]
[2] Sarno R et al (2002) Complete nucleotide sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1. Antimicrob Agents Chemother. 46(11):3422-7. [PMID:12384346]
 N/A
 N/A
Information of plasmid
ID 196
Plasmid name pJHCMW1
GenBank accession number NC_003486.1
Genome size 11354 bp
Coordinate of oriT  [Strand] 1883..2100 [-]
Drug resistance experimental resistance to amikacin (aac(6 )-Ib), tobramycin (aac(6 )-Ib), kanamycin (aac(6 )-Ib);

presence of aadA1 (aminoglycoside adenylyltransferase), blaOXA-9 (Beta-Lactam, bla OXA-9), and blaTEM-1 (beta-lactamase CTX-M-14)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae [573]
Reference
[1] Dery KJ et al (1997) Characterization of the replication and mobilization regions of the multiresistance Klebsiella pneumoniae plasmid pJHCMW1. Plasmid. 38(2):97-105. [PMID:9339467]
[2] Sarno R et al (2002) Complete nucleotide sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1. Antimicrob Agents Chemother. 46(11):3422-7. [PMID:12384346]