 oriTDB accession number | 100202 |
| Name | oriT_pCI528  |
Sequence Completeness  | intact |
| oriT length | 171 nt |
| oriT sequence | GATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGTGTAAAGCAAATCG ACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGT AATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTA |
| IR (inverted repeat) | IR1: [10-19] [24-33] nt (CCCATTTTAT..ATAAAATGGG) IR2: [79-91] [95-107] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [113-127] [140-154] nt (CACATTGTAATACAA..TTGTATTACAATGTG) |
| Location of nic site | [162-163] nt |
| Conserved sequence flanking the nic site | CTTG|CA |
| Note | _ |
Visualization of oriT structure Reference| [1] Emond E et al (2001) Molecular characterization of a theta replication plasmid and its use for development of a two-component food-grade cloning system for Lactococcus lactis. Appl Environ Microbiol. 67(4):1700-9. [PMID:11282624] |
| ID | 195 |
| Name | Orf2_pCI528 |
| GenBank accession number | AAB28188 |
| Family | MOBP |
| Length | 410 aa |
| UniProt ID | Q79DL9 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 5.20E-75, Aligned region: 9..280 |
| Note | relaxase |
| Protein sequence [Download] | MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKRTKQNEFSAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL |
Reference| # | ID | Name | GenBank | Length | Note |
| 1 | 275 | _ | _ | _ | _ |
| ID | 195 |
| Plasmid name | pCI528 |
| GenBank accession number | L08601.1 |
| Genome size | 46 kb |
| Coordinate of oriT [Strand] | _ |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactococcus lactis subsp. cremoris UC503 [1359] |
Reference