oriTDB

The information of the oriT region

oriTDB accession number	100201
Name	oriT_pDR1-1
Sequence Completeness	intact
oriT length	335 nt
oriT sequence	GATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCG ACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGT AATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTAT ATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCA ATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGTTCTTGA TGGATCAGAAAAATGTATCACAAATTTAAACAAAG
IR (inverted repeat)	IR1: [10-19] [24-33] nt (CCCATTTTAT..ATAAAATGGG) IR2: [79-91] [95-107] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [113-127] [140-154] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[162-163] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	_
	L:10-19;R:24-33;L:79-91;R:95-107;L:113-127;R:140-154;D:162-163

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAAAGCAAATCG

61

ACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGT

121

AATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTAT

181

ATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATTACAAAATCA

241

ATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAAGTTCTTGA

301

TGGATCAGAAAAATGTATCACAAATTTAAACAAAG

Reference

[1] Strahinic I et al (2009) Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp. lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. J Appl Microbiol. 106(1):78-88. [PMID:19040703]

N/A

Information of plasmid

ID	194
Plasmid name	pDR1-1
GenBank accession number	NC_004164.2
Genome size	7412 bp
Coordinate of oriT [Strand]	2..336 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis lactis bv. diacetylactis DRC1 [44688]

Reference
N/A