oriTDB

The information of the oriT region

oriTDB accession number	100199
Name	oriT_pKJ36
Sequence Completeness	core
oriT length	32 nt
oriT sequence	ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT
IR (inverted repeat)	[1-8] [12-19] nt (ATGCAACC..GGTTGCAT)
Location of nic site	[28-29] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	_
	L:1-8;R:12-19;D:28-29

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATGCAACCTCCGGTTGCATGTAAGTGCGCCCT

Reference

[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

[2] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]

I. Information of Relaxase

ID	192
Name	MobB_pKJ36
GenBank accession number	NP_072178
Family	MOB_Q
Length	346 aa
UniProt ID	Q9F152
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 3.50E-66, Aligned region: 17..254
Note	MobA/MobL family; 39.0 kDa; pI=10.66; similar to plasmid pKJ50 MobA protein
Protein sequence [Download]	MAIYHLSVSNVSRASGSRATATLSYITGKRVHDERRGETYDYGRKERVLRVGTLLPEGAP AEFADPAVLFNAVELHETGRTARPAKKIVVALPREFTPRQRVQALEEYIRENLNADGYAA TYAIHEDREGNNPHAHILVANRQIDPATGGWARLKQRMEYVLDERGERVPLIDPETGRQK TDKRGRRQWKRTSVSLNPLDRKAKLKALRESWAKTCNARLDETARIDHRSLEDQGSDLEP TIHEGYAARAIERAGGVSERCEANREIRRSNGLLTAIRTELGRIFDRLGELFAAKIRQLR QRQAERNRPRSRTGATSRATRAASSKPTGPTTRQRSVENSWEPRPT

Reference

[1] Francia MV et al (2004) A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100. [PMID:14975531]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	272	_	_	_	_

N/A

Information of plasmid

ID	192
Plasmid name	pKJ36
GenBank accession number	NC_002635.1
Genome size	3625 bp
Coordinate of oriT [Strand]	3482..3513 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum KJ [216816]

Reference
N/A