oriTDB
The information of the oriT region
oriTDB accession number 100198
Name oriT_pRE25  insolico
Sequence Completeness core
oriT length 38 nt
oriT sequence ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT
IR (inverted repeat)[1-12] [16-27] nt  (ATACGAAGTAAC..GTTACTGCGTAT)
Location of nic site [33-34] nt
Conserved sequence flanking the
  nic site 		TAAGTGC|GCCCT
Note The oriT is identical to oriT_pIP501
L:1-12;R:16-27;D:33-34
Visualization of oriT structure
Reference
[1] Schwarz FV et al (2001) Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid . 46(3):170-87. [PMID:11735367]
[2] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]
I. Information of Relaxase
ID 191
Name Orf24_pRE25 insolico
GenBank accession number CAC29179
Family MOBQ
Length 654 aa
UniProt ID Q79A51
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 2.10E-66, Aligned region: 11..235
Note relaxase
Protein sequence [Download] MTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDR
QTLWNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHR
DDENNPHAHIMLTMREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRK
SLVSEIRKDWADKVNQYLKDRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISE
LKRKNLEIQSYNDILAELDKLENQEKVLKQDQNFTLKFEKTFSPLEKGELKNLSKELKLF
INDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFF
KKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPF
QTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVS
IDSVIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQ
GVFFVQDSMKHIDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDV
PEKNEVTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
Reference
[1] Schwarz FV et al (2001) Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid . 46(3):170-87. [PMID:11735367]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 271 _ insolico _ _ _
 N/A
Information of plasmid
ID 191
Plasmid name pRE25
GenBank accession number X92945.2
Incompatibility group Inc18
Genome size 50237 bp
Coordinate of oriT  [Strand] 19481..19518 [-]
Drug resistance experimental resitance to chloramphenicol, MLS (macrolide-lincosamide-streptogramin B)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecalis RE25 [1351]
Reference
[1] Schwarz FV et al (2001) Sequence of the 50-kb conjugative multiresistance plasmid pRE25 from Enterococcus faecalis RE25. Plasmid . 46(3):170-87. [PMID:11735367]

Microbial Bioinformatics Group in MML, SJTU