 oriTDB accession number | 100197 |
| Name | oriT_pMRC01  |
Sequence Completeness  | core |
| oriT length | 38 nt |
| oriT sequence | ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT |
| IR (inverted repeat) | [1-11] [14-25] nt (ACACCACCCAA..TTGGAGTGGTGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | TAAGTGCGCATT |
| Note | The oriT region was determined experimentally, but the putative nic site was not determined experimentally. |
Visualization of oriT structure (The oriT was characterized experimentally) Reference| [1] Dougherty BA et al (1998) Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147. Mol Microbiol. 29(4):1029-38. [PMID:9767571] |
| [2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398] |
| [3] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207] |
| [4] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691] |
| ID | 190 |
| Name | TraA_pMRC01  |
| GenBank accession number | NP_047290 |
| Family | MOBQ |
| Length | 680 aa |
| UniProt ID | O87207 |
| PDB ID | _ |
| Pfam | MobA_MobL [PF03389.14], Evalue: 1.10E-78, Aligned region: 17..240 |
| Note | relaxase |
| Protein sequence [Download] | MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYNRSVMPESFILLPENAPE WAKDRQKLWNEVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVAD VAIHRDHDENPHAHVMLTNRPFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLV DWDKVGKVEEWRKAWADHVNSVFQEKNIDERISEKTLEAQGINDIATQHVGVTGNRDERA EFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNEKKVIAELSKELHTFIDLEHL EEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSRQEFSLDNANRLIDKVVDRSIKAMYPTV DSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEV RKIVEQQYSVVLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPI LFSTKEHNQGLSYLAGRIRLEDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEAT NRNDKQSYKNEFYRDKNMENYQSVAYSDTSPAEYMNRLFSGVLISVLYNNENQHKKGLEE VEWDMERKHREHTKTGGLSR |
Reference| [1] Dougherty BA et al (1998) Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147. Mol Microbiol. 29(4):1029-38. [PMID:9767571] |
| [2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398] |
| [3] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 270 | _ | _ | _ | _ |
| ID | 190 |
| Plasmid name | pMRC01 |
| GenBank accession number | NC_001949.1 |
| Incompatibility group | IncQ-type |
| Genome size | 60232 bp |
| Coordinate of oriT [Strand] | 1811..1848 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactococcus lactis DPC3147 [1358] |
Reference