oriTDB

The information of the oriT region

oriTDB accession number	100197
Name	oriT_pMRC01
Sequence Completeness	core
oriT length	38 nt
oriT sequence	ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT
IR (inverted repeat)	[1-11] [14-25] nt (ACACCACCCAA..TTGGAGTGGTGT)
Location of nic site	_
Conserved sequence flanking the nic site	TAAGTGCGCATT
Note	The oriT region was determined experimentally, but the putative nic site was not determined experimentally.
	L:1-11;R:14-25

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT

Reference

[1] Dougherty BA et al (1998) Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147. Mol Microbiol. 29(4):1029-38. [PMID:9767571]

[2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

[3] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]

[4] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]

I. Information of Relaxase

ID	190
Name	TraA_pMRC01
GenBank accession number	NP_047290
Family	MOB_Q
Length	680 aa
UniProt ID	O87207
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 1.10E-78, Aligned region: 17..240
Note	relaxase
Protein sequence [Download]	MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYNRSVMPESFILLPENAPE WAKDRQKLWNEVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVAD VAIHRDHDENPHAHVMLTNRPFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLV DWDKVGKVEEWRKAWADHVNSVFQEKNIDERISEKTLEAQGINDIATQHVGVTGNRDERA EFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNEKKVIAELSKELHTFIDLEHL EEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSRQEFSLDNANRLIDKVVDRSIKAMYPTV DSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEV RKIVEQQYSVVLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPI LFSTKEHNQGLSYLAGRIRLEDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEAT NRNDKQSYKNEFYRDKNMENYQSVAYSDTSPAEYMNRLFSGVLISVLYNNENQHKKGLEE VEWDMERKHREHTKTGGLSR

Reference

[1] Dougherty BA et al (1998) Sequence and analysis of the 60 kb conjugative, bacteriocin-producing plasmid pMRC01 from Lactococcus lactis DPC3147. Mol Microbiol. 29(4):1029-38. [PMID:9767571]

[2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

[3] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	270	_	_	_	_

N/A

Information of plasmid

ID	190
Plasmid name	pMRC01
GenBank accession number	NC_001949.1
Incompatibility group	IncQ-type
Genome size	60232 bp
Coordinate of oriT [Strand]	1811..1848 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis DPC3147 [1358]

Reference
N/A