oriTDB

The information of the oriT region

oriTDB accession number	100196
Name	oriT_pKJ50
Sequence Completeness	core
oriT length	35 nt
oriT sequence	TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT
IR (inverted repeat)	[4-11] [15-22] nt (ATGTTACC..GGTAACAT)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	_
	L:4-11;R:15-22;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGAATGTTACCACCGGTAACATGTAAGTGCGCCCT

Reference

[1] Park MS et al (1999) Sequence analysis of plasmid pKJ50 from Bifidobacterium longum. Microbiology. 145 ( Pt 3):585-92. [PMID:10217492]

[2] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

[3] Becker EC et al (2003) Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids. J Bacteriol. 185(12):3538-46. [PMID:12775691]

I. Information of Relaxase

ID	189
Name	MobA_pKJ50
GenBank accession number	AAD00257
Family	MOB_Q
Length	345 aa
UniProt ID	Q9ZA19
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 7.80E-34, Aligned region: 60..224
Note	relaxase
Protein sequence [Download]	MRDERTGEAFNGFGRRSASSMYARCCPRARPASTSTRSVCSTPSRWPRNVPTRGPRRRSW SPCPASSTPANAFRALEDFISWNITANGYACTYAIHTDKDGRNPHAHILVANRRIDPKTG RWAAKSRSEFALDANGRRIPVIDPDTGRQKIGARNRKVWKRVNVSNNPLDSKEFLERLRR EWADSCNALLPGYAVIDHRSFKARGIERIPTIHEGYASREMEKRGGRGDLCEENRRIQAL NRLLDALRAMIGRLSDQAQGILTAVKRRQRPSGAPQAPSSPEPAERPQERPQARETPPQA PSSPEPAERPQERPQARETPATGPAGTPEAHQDEKGAHRQVQDTA

Reference

[1] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	269	_	_	_	_

N/A

Information of plasmid

ID	189
Plasmid name	pKJ50
GenBank accession number	NC_004978.1
Genome size	4960 bp
Coordinate of oriT [Strand]	2462..2496 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bifidobacterium longum KJ [216816]

Reference
N/A