oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100195
Name	oriT_pIJB1
Sequence Completeness	intact
oriT length	99 nt
oriT sequence	GAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCTAACTTCACACATCCTG CCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACA
IR (inverted repeat)	[13-31] [34-52] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[60-61] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	_
	L:13-31;R:34-52;D:60-61

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	188
Name	TraI_pIJB1
GenBank accession number	YP_006965894
Family	MOB_P
Length	730 aa
UniProt ID	K4IYE5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.30E-56, Aligned region: 12..252
Note	TraI Conjugative transfer protein, DNA relaxase
Protein sequence [Download]	MIAKHVSMKTVQKSDFAGLVKYITDEQAKNERVGYVAVTNCHTDRPEVAITEVLNTQAQN TRSGADKTYHLIVSFRPGEQPDDATLKAIEARICEGLGFGEHQRVSAVHHDTDNLHIHIA INKIHPTRYTILEPFNAYHTLGQLCEKLEREYGLERDNHQGNKLVSEGRAQDMERHAGVE SLQGWVKRECAADMQAAQSWEALHQVMRDNGLELRERGNGFVIQAADGLAVKASSIGREF SKAKLEERLGTFEPSAEHLERAAQKPRKQYDKKPVRSRVNTVELHAKYKADQLTISGSRT AEWEKARAKKDRLIEAAKRSGRLKRSTIKLISGAGVGKKLMYSATSKTMKSEIEKINKQY LKERQEIHEKYQRRAWADWLRAKAAEGDQEALGALRARDAAQGLKGNTVAGTGPLRPQAA AAEQDSVTKKGTIIYRVGPTAVRDDGDRLKVSRGADQDGMQAALRMAMDRYGDRISVGGT PAFKEQIAQAAAASKLPITFDDDALERRRQELLTQATTKENDHDRTDRGRAAGGGISGSG PAIAAIPGAARAGQPRAAAAGRTTKPNVGRVGRKPPPQSQNRLRRLSQLGVVQLASGSEV LLPRHVPGDVEQQGAKPVDGLRRDIFGPGRLSPQVAAAADKYIAEREGKRLKGFDIPKHS RYTDGQDGAATFAGIRQVEGQSLALLTRGEEVMVLPVDEPTARRLKRLAVGDAITVTPQG SIKTTKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	266	TraH_pIJB1	YP_006965895	108 aa	TraH Conjugative transfer protein, relaxosome stabilization
2	267	TraJ_pIJB1	YP_006965896	129 aa	TraJ Conjugative transfer protein, relaxosome protein
3	268	TraK_pIJB1	YP_006965897	132 aa	TraK conjugative transfer protein,oriT binding protein

N/A

Information of plasmid

ID	188
Plasmid name	pIJB1
GenBank accession number	NC_019378.1
Incompatibility group	IncP-1
Genome size	99001 bp
Coordinate of oriT [Strand]	43131..43230 [+]
Drug resistance	_
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	contains tfd genes for 2,4-D degradation
Host bacterium [NCBI Taxonomy ID]	Burkholderia cepacia 2a [292]

Reference

[1] Poh RP et al (2002) Complete characterisation of Tn5530 from Burkholderia cepacia strain 2a (pIJB1) and studies of 2,4-dichlorophenoxyacetate uptake by the organism. Plasmid. 48(1):1-12. [PMID:12206751]

[2] Xia XS et al (1998) A novel plasmid pIJB1 possessing a putative 2,4-dichlorophenoxyacetate degradative transposon Tn5530 in Burkholderia cepacia strain 2a. Plasmid. 39(2):154-9. [PMID:9514710]