oriTDB

The information of the oriT region

oriTDB accession number	100194
Name	oriT_pTB19
Sequence Completeness	core
oriT length	41 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGCTATACTTTACATG
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	TAGTGTG\|CTAT
Note	_
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGCTATACTTTACATG

Reference

[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]

I. Information of Relaxase

ID	187
Name	Mob_pTB19
GenBank accession number	AAA98305
Family	MOB_V
Length	415 aa
UniProt ID	Q03987
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDLLESELKNKKSELAILSEEVSGEFKIPVKREKKSVEVPT GKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKQKVKAGAVLQTRVDQLLNTDYAKENQSL KKEVKELRSTNKSLSEENGRLKSTVEHLTNEIESLYVLTKDFLKARTNDLESFKELFGVF VGKVKEKAPRGLFVRQHERSEEKKNTFSLQDVLQRDRELREQRKAKRKKSHDLER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	265	_	_	_	_

N/A

Information of plasmid

ID	187
Plasmid name	pTB19
GenBank accession number	M63891.1
Genome size	11887 bp
Coordinate of oriT [Strand]	9869..9909 [+]
Drug resistance	resistance to tetracycline, kanamycin, bleomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Geobacillus stearothermophilus [1422]

Reference

[1] Oskam L et al (1991) The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions. Plasmid. 26(1):30-9. [PMID:1946749]