oriTDB
The information of the oriT region
oriTDB accession number 100194
Name oriT_pTB19  insolico
Sequence Completeness core
oriT length 41 nt
oriT sequence CACTTTATGAATATAAAGTATAGTGTGCTATACTTTACATG
IR (inverted repeat)[2-8] [13-19] nt  (ACTTTAT..ATAAAGT)
Location of nic site [27-28] nt
Conserved sequence flanking the
  nic site 		TAGTGTG|CTAT
Note _
L:2-8;R:13-19;D:27-28
Visualization of oriT structure
Reference
[1] Grohmann E et al (2003) Conjugative plasmid transfer in gram-positive bacteria. Microbiol Mol Biol Rev. 67(2):277-301. [PMID:12794193]
I. Information of Relaxase
ID 187
Name Mob_pTB19 insolico
GenBank accession number AAA98305
Family MOBV
Length 415 aa
UniProt ID Q03987
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDLLESELKNKKSELAILSEEVSGEFKIPVKREKKSVEVPT
GKRNLLGIEQKKTVMKSTGNVILKDEVFQDLKQKVKAGAVLQTRVDQLLNTDYAKENQSL
KKEVKELRSTNKSLSEENGRLKSTVEHLTNEIESLYVLTKDFLKARTNDLESFKELFGVF
VGKVKEKAPRGLFVRQHERSEEKKNTFSLQDVLQRDRELREQRKAKRKKSHDLER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 265 _ insolico _ _ _
 N/A
Information of plasmid
ID 187
Plasmid name pTB19
GenBank accession number M63891.1
Genome size 11887 bp
Coordinate of oriT  [Strand] 9869..9909 [+]
Drug resistance insolico resistance to tetracycline, kanamycin, bleomycin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Geobacillus stearothermophilus [1422]
Reference
[1] Oskam L et al (1991) The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions. Plasmid. 26(1):30-9. [PMID:1946749]

Microbial Bioinformatics Group in MML, SJTU